From Exogenous to Endogenous: The Inevitable Imprint of Mass Spectrometry in Metabolomics

Department of Molecular Biology, The Scripps Center for Mass Spectrometry, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Journal of Proteome Research (Impact Factor: 4.25). 03/2007; 6(2):459-68. DOI: 10.1021/pr060505+
Source: PubMed


Mass spectrometry (MS) is an established technology in drug metabolite analysis and is now expanding into endogenous metabolite research. Its utility derives from its wide dynamic range, reproducible quantitative analysis, and the ability to analyze biofluids with extreme molecular complexity. The aims of developing mass spectrometry for metabolomics range from understanding basic biochemistry to biomarker discovery and the structural characterization of physiologically important metabolites. In this review, we will discuss the techniques involved in this exciting area and the current and future applications of this field.

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    • "FO-BEG1 into the medium during growth and to evaluate the effect of phosphate limitation on them, we performed an ultra-high resolution mass spectrometry analysis of the bacterial exo-metabolome. Mass spectrometry is the most widely used approach in metabolomic studies [14]. In particular high resolution accurate mass (HRAM) mass spectrometry instruments are receiving progressively more attention, owing to their ability to resolve highly complex samples and to yield accurate mass measurements, which allow precise calculations of the elemental composition [15], [16], [18]. "
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    PLoS ONE 05/2014; 9(5):e96038. DOI:10.1371/journal.pone.0096038 · 3.23 Impact Factor
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    • "Review: bridge between chemistry and biology Kikuchi and Kakeya (2006) Bioinfo: metabolome platform in FT-ICR-MS. Metabolite accumulation in herbicidal modes of action Arabidopsis thaliana Oikawa et al. (2006) Bioinfo: taxonomic diversity of flavonoids Shinbo et al. (2006b) DB: chemical biology Tomiki et al. (2006) Bioinfo: MS peak storage and processing Gaida and Neumann (2007) Review: GC-MS DB Hummel et al. (2007) Exp: metabolite accumulation patterns caused by herbicidal enzyme inhibitors Arabidopsis thaliana Ohta et al. (2007) Review: metabolomics technologies Moco et al. (2007) Review: functional genomics research strategy of combining transcriptome and metabolome Saito et al. (2007) Exp: assignment of UGT89C1 to a flavonol 7-O-rhamnosyltransferase Arabidopsis thaliana Yonekura-Sakakibara et al. (2007) Review: the role of MS in metabolomics Want et al. (2007) Exp: light/dark regulation of metabolite activities Arabidopsis thaliana Nakamura et al. (2007) DB: MS DB Akiyama et al. (2008) Exp: characterization of mutants in flavonoid and phenylpropanoid biosynthetic pathways Arabidopsis thaliana Böttcher et al. (2008) Review: MS platforms Dunn et al. (2008) Exp: metabolism of dietary phytochemicals Rat Fardet et al. (2008) Exp: metabolic networks in primary and secondary pathways for achene and receptacle Fragaria ananassa Fait et al. (2008) Exp: a combination of high-resolution mass spectrometry and 13 C-isotope labeling of entire metabolomes Giavalisco et al. (2008) Review: metabolomics technologies and functional genomics platform Papaver somniferum Hagel and Facchini (2008) Exp: phenolic biosynthesis pathway FragariaÂananassa Hanhineva et al. (2008) Exp: metabolic profiling in late stages of strawberry receptacle development FragariaÂananassa Hanhineva (2008) Bioinfo and DB: metabolite annotation based on MS Solanum lycopersicum Iijima et al. (2008) Exp: regulation of glucosinolate biosynthesis by two clades of regulators Arabidopsis thaliana Malitsky et al. (2008) Bioinfo: identification of metabolites based on MS and MS-tagged MS 2 data Arabidopsis thaliana Matsuda et al. (2008) Exp: integrated analysis of metabolome and transcriptome Solanum lycopersicum Mintz-Oron et al. (2008) Review: technology and informatics Oryza sativa Oikawa et al. (2008) Exp: protocol in metabolite fingerprints Overy et al. (2008) Bioinfo: metabolome platform in FT-ICR-MS. Determination of growth-specific metabolites Escherichia coli Takahashi et al. (2008) Review: atmospheric pressure ionization MS Werner et al. (2008) Exp: annotation of metabolite information to MS Glycine max Ara et al. (2009) DB: embedded string-search commands on MediaWiki Arita and Suwa (2009) Exp: metabolic profiling in cold temperature Arabidopsis lyrata Davey et al. (2009) Bioinfo: tools for the annotation of high-resolution MS metabolomics data Draper et al. (2009) Review: integrated OMICs Fukushima et al. (2009) Review: MS-based technologies Han et al. (2009) Exp: changes in antioxidant compounds in white cabbage during winter storage Brassica oleracea var. "
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