Article

Hematopoietic stem cell aging: Mechanism and consequence

Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Experimental Gerontology (Impact Factor: 3.53). 06/2007; 42(5):385-90. DOI: 10.1016/j.exger.2006.11.019
Source: PubMed

ABSTRACT Advancing age is frequented by the onset of a variety of hematological conditions characterized by diminished homeostatic control of blood cell production. The fact that upstream hematopoietic stem and progenitor cells are obligate mediators of homeostatic control of all blood lineages, has implicated the involvement of these cells in the pathophysiology of these conditions. Indeed, evidence from our group and others has suggested that two of the most clinically significant age-associated hematological conditions, namely, the diminution of the adaptive immune system and the elevated incidence of myeloproliferative diseases, have their origin in cell autonomous changes in the functional capacity of hematopoietic stem cells.

0 Followers
 · 
81 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: A comparative analysis of the numbers of fibroblast colony-forming (CFC-F) and granulocyte-macrophage colony-forming (CFC-GM) progenitor cells in the bone marrow, as well as the thymulin and melatonin content in the blood of young and old mice of the CBA/Ca and FVB/N strains was carried out. Only a tendency toward an increasing amount of CFC-F and CFC-GM cells in the bone marrow of aging CBA/Ca mice was observed and these indices changed significantly in FVB/N mice. The strain-dependent differences of the age-related changes in the biological properties of the cellular composition of the bone marrow are largely associated with the peculiarities of relationships between the pineal gland and thymus functions in mice of different strains with aging.
    04/2014; 4(2):134-139. DOI:10.1134/S2079057014020118
  • [Show abstract] [Hide abstract]
    ABSTRACT: Stem cells persist in replenishing functional mature cells throughout life by self-renewal and multilineage differentiation. Hematopoietic stem cells (HSCs) are among the best-characterized and understood stem cells, and they are responsible for the life-long production of all lineages of blood cells. HSCs are a heterogeneous population containing lymphoid-biased, myeloid-biased, and balanced subsets. HSCs undergo age-associated phenotypic and functional changes, and the composition of the HSC pool alters with aging. HSCs and their lineage-biased subfractions can be identified and analyzed by flow cytometry based on cell surface makers. Fluorescence-activated cell sorting (FACS) enables the isolation and purification of HSCs that greatly facilitates the mechanistic study of HSCs and their aging process at both cellular and molecular levels. The mouse model has been extensively used in HSC aging study. Bone marrow cells are isolated from young and old mice and stained with fluorescence-conjugated antibodies specific for differentiated and stem cells. HSCs are selected based on the negative expression of lineage markers and positive selection for several sets of stem cell markers. Lineage-biased HSCs can be further distinguished by the level of SLAM/CD150 expression and the extent of Hoechst efflux.
    Methods in molecular biology (Clifton, N.J.) 01/2015; 1235:19-32. DOI:10.1007/978-1-4939-1785-3_3 · 1.29 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The elucidation of the molecular mechanisms underlying the effects of traditional Chinese medicines in clinical practice is a key step toward their worldwide application, and this topic is currently a subject of intense research interest. Rg1, a component of ginsenoside, has recently been shown to perform several pharmacological functions; however, the underlying mechanisms of these effects remain unclear. In the present study, we investigated whether Rg1 has an anti-senescence effect on hematopoietic stem cells (HSCs) and the possible molecular mechanisms driving any effects. The results showed that Rg1 could effectively delay tert-butyl hydroperoxide (t-BHP)-induced senescence and inhibit gene expression in the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in HSCs. Our study suggested that these two signaling pathways might be potential targets for elucidating the molecular mechanisms of the Rg1 anti-senescence effect.
    Genetics and molecular research: GMR 01/2014; 13(4):10086-10096. DOI:10.4238/2014.December.4.3 · 0.85 Impact Factor

Preview

Download
0 Downloads
Available from