Drosophila melanogaster Thor and Response to Candida albicans Infection

Genetics Group, Biotechnology Research Institute, National Research Council, Montreal, Quebec H4P 2R2, Canada.
Eukaryotic Cell (Impact Factor: 3.18). 05/2007; 6(4):658-63. DOI: 10.1128/EC.00346-06
Source: PubMed


We used Drosophila melanogaster macrophage-like Schneider 2 (S2) cells as a model to study cell-mediated innate immunity against infection by the opportunistic
fungal pathogen Candida albicans. Transcriptional profiling of S2 cells coincubated with C. albicans cells revealed up-regulation of several genes. One of the most highly up-regulated genes during this interaction is the D. melanogaster translational regulator 4E-BP encoded by the Thor gene. Analysis of Drosophila 4E-BPnull mutant survival upon infection with C. albicans showed that 4E-BP plays an important role in host defense, suggesting a role for translational control in the D. melanogaster response to C. albicans infection.

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    • "A high-throughput screen in Drosophila melanogaster S2 RNAi library identified a novel protein, macroglobulin complement related (Mcr), that exerts opsonizing activity specifically against Candida albicans [33]. In addition, S2 Drosophila cells efficiently eliminate C. albicans yeast cells and induce significant damage to the hyphae of filamentous fungi, including Aspergillus and the Mucorales, in a way that resembles the antifungal effector function of human phagocytes [23, 34]. "
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    International Journal of Microbiology 02/2012; 2012:583792. DOI:10.1155/2012/583792
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    • "Also, in addition to causing tissue damage, the induced expression of Sap2 may lead to the degradation of extracellular signaling components important for energy homeostasis or the host immune response like secreted antimicrobial peptides (AMPs). In fact, it has been shown that Drosophila genes involved in protein translation, energy homeostasis, and stress responses are important for the host to survive an infection [57], [58], [59]. Thus, while it is likely that less tissue damage caused by infection is the primary reason flies survive infection with stp1Δ compared to wild type C. albicans, it is possible that stp1Δ mutants may fail to interfere with the other facets of the host's ability to survive infection. "
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    PLoS ONE 11/2011; 6(11):e27434. DOI:10.1371/journal.pone.0027434 · 3.23 Impact Factor
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    • "Using the live Drosophila model, Thor was found to be involved in fly survival in response to Candida infection, suggesting a significant component of the fruit fly's cell-based immunity may involve regulation of translation (Fig. 2) (Levitin et al., 2007). This validation of the results derived from Drosophila macrophage-like cells by using the whole fly helps to confirm S2 cells as a useful model to study Drosophila– Candida interactions (Levitin et al., 2007). "
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