Article

Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA.
Applied Optics (Impact Factor: 1.78). 03/2007; 46(6):993-1000. DOI: 10.1364/AO.46.000993
Source: PubMed

ABSTRACT We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.

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    • "Therefore, the use of this technique is limited only to those applications in which the observed targets can be illuminated by a laser. Synthetic aperture carried out by a combination of several off-axis incoherent holograms in scanning holographic microscopy has been demonstrated by (Indebetouw et al., 2007). However, this method is limited to microscopy only, and although it is a technique of recording incoherent holograms, a specimen should also be illuminated by an interference pattern between two laser beams. "
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    Holography, Research and Technologies, 02/2011; , ISBN: 978-953-307-227-2
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    • "Finally, all the propagated distributions are used to assemble a synthetic expanded aperture that yields a superresolved image by Fourier transformation. Thus, and in summary, the whole proposed procedure could be understood as a technique based on time multiplexing the spatial-frequency content diffracted by the input sample in a similar way to that which sequential off-axis illumination performs in digital holographic microscopy [29] [30] [31] [32] [33] [34] [35] [36], but where the synthetic aperture is generated by CCD displacement [23] [24] [25] [26] [27] [28]. "
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