Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA.
Applied Optics (Impact Factor: 1.78). 03/2007; 46(6):993-1000. DOI: 10.1364/AO.46.000993
Source: PubMed

ABSTRACT We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.

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Available from: Joseph Rosen, Aug 16, 2015
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    • "Therefore, the use of this technique is limited only to those applications in which the observed targets can be illuminated by a laser. Synthetic aperture carried out by a combination of several off-axis incoherent holograms in scanning holographic microscopy has been demonstrated by (Indebetouw et al., 2007). However, this method is limited to microscopy only, and although it is a technique of recording incoherent holograms, a specimen should also be illuminated by an interference pattern between two laser beams. "
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    ABSTRACT: Holographic imaging offers a reliable and fast method to capture the complete threedimensional (3D) information of the scene from a single perspective. However, holography is not widely applied to the regime of white-light imaging, because white-light is incoherent and in general creating holograms requires a coherent interferometer system. In this chapter
    Holography, Research and Technologies, 02/2011; , ISBN: 978-953-307-227-2
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    • "Finally, all the propagated distributions are used to assemble a synthetic expanded aperture that yields a superresolved image by Fourier transformation. Thus, and in summary, the whole proposed procedure could be understood as a technique based on time multiplexing the spatial-frequency content diffracted by the input sample in a similar way to that which sequential off-axis illumination performs in digital holographic microscopy [29] [30] [31] [32] [33] [34] [35] [36], but where the synthetic aperture is generated by CCD displacement [23] [24] [25] [26] [27] [28]. "
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    ABSTRACT: Holography in the Gabor regime is restricted to weak diffraction assumptions. Otherwise, diffraction prevents an accurate recovery of the object's complex wavefront. We have recently proposed a modified Gabor-like setup to extend Gabor's concept to any sample provided that it be non-diffusive. However, the resolution of the final image becomes limited as a consequence of the additional elements considered in the proposed setup. In this paper we present an experimental approach to overcome such a limitation in which the former configuration is used while the CCD camera is shifted to different off-axis positions in order to generate a synthetic aperture. Thus, once the whole image set is recorded and digitally processed for each camera position, we merge the resulting band-pass images into one image by assembling a synthetic aperture. Finally, a superresolved image is recovered by Fourier transformation of the information contained in the generated synthetic aperture. Experimental results validate our concepts for a gain in resolution of close to 2.
    Journal of Optics A Pure and Applied Optics 09/2009; 11(12):125408. DOI:10.1088/1464-4258/11/12/125408 · 1.92 Impact Factor
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    • "[25] "
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    ABSTRACT: The use of tilted illumination onto the input object in combination with time multiplexing is a useful technique to overcome the Abbe diffraction limit in imaging systems. It is based on the generation of an expanded synthetic aperture that improves the cutoff frequency (and thus the resolution limit) of the imaging system. In this paper we present an experimental validation of the fact that the generation of a synthetic aperture improves not only the lateral resolution but also the axial one. Thus, it is possible to achieve higher optical sectioning of three-dimensional (3D) objects than that defined by the theoretical resolution limit imposed by diffraction. Experimental results are provided for two different cases: a synthetic object (micrometer slide) imaged by a 0.14 numerical aperture (NA) microscope lens, and a biosample (swine sperm cells) imaged by a 0.42 NA objective.
    Journal of Optics A Pure and Applied Optics 12/2008; 10(12). DOI:10.1088/1464-4258/10/12/125001 · 1.92 Impact Factor
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