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How does arbuscular mycorrhizal symbiosis regulate root hydraulic properties and plasma membrane aquaporins in Phaseolus vulgaris under drought, cold or salinity stresses? New Phytol

Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C/Profesor Alabareda 1, 18008, Granada, Spain.
New Phytologist (Impact Factor: 7.67). 02/2007; 173(4):808-16. DOI: 10.1111/j.1469-8137.2006.01961.x
Source: PubMed

ABSTRACT Here, we evaluated how the arbuscular mycorrhizal (AM) symbiosis regulates root hydraulic properties and root plasma membrane aquaporins (PIP) under different stresses sharing a common osmotic component. Phaseolus vulgaris plants were inoculated or not with the AM fungus Glomus intraradices, and subjected to drought, cold or salinity. Stress effects on root hydraulic conductance (L), PIP gene expression and protein abundance were evaluated. Under control conditions, L in AM plants was about half that in nonAM plants. However, L was decreased as a result of the three stresses in nonAM plants, while it was almost unchanged in AM plants. At the same time, PIP2 protein abundance and phosphorylation state presented the same trend as L. Finally, the expression of each PIP gene responded differently to each stress and was dependent on the AM fungal presence. Differential expression of the PIP genes studied under each stress depending on the AM fungal presence may indicate a specific function and regulation by the AM symbiosis of each gene under the specific conditions of each stress tested.

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Available from: Juan Manuel Ruiz-Lozano, Oct 29, 2014
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    • "In general, plants supply associated AM fungi with carbohydrates, and in return, fungi provide soil phosphorus (P) and possibly nitrogen (N) to their host plants (Hodge et al., 2010; Selosse and Rousset, 2011). Mycorrhizal symbioses can also provide plants with other benefits such as protection against root pathogens (Lewandowski et al., 2013) and several types of abiotic stress (Aroca et al., 2007). Furthermore, increasing evidence shows that mycorrhizas influence the structure of plant communities (Klironomos et al., 2011; Yang et al., 2014), the rhizosphere microbiome (Vestergård et al., 2008; Veresoglou et al., 2012), soil structure (van der Heijden et al., 2006; Leifheit et al., 2015), and nutrient cycles (Cheng et al., 2012; Bender et al., 2015). "
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    Soil Biology and Biochemistry 10/2015; 89:196-205. DOI:10.1016/j.soilbio.2015.07.007 · 4.41 Impact Factor
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    • "cognizes the phosphorylation of PIP2 proteins in a serine residue in loop B . All the antibodies were designed against the most conservative regions of these aquaporin groups ( Calvo - Polanco et al . 2014 ) . To detect PIP1 aquaporins , we used the first 26 amino acids of the N - terminal part of the PvPIP1 ; 3 protein ( accession No . DQ855475 ; Aroca et al . 2007 ) , raised as a peptide to immunize mice . To detect PIP2 aquaporins , we used the last 12 amino acids of the C - terminal part of the PvPIP2 ; 1 protein ( accession No . AY995195 ; Aroca et al . 2006 ) , raised as a peptide to immunize rabbits . To detect phosphorylated PIP2 , we used the same protein PvPIP2 ; 1 as the amino acid seque"
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    Plant Cell and Environment 01/2015; 38(8). DOI:10.1111/pce.12507 · 5.91 Impact Factor
    • "Author's personal copy (Chaumont & Tyerman, 2014; Murai-Hatano et al., 2008), plant growth, stress responses (Vandeleur et al., 2009), and regulation of plant–water relations (Maurel et al., 2008). They are also important in symbiotic relationship such as plant–fungi interactions (Aroca, Porcel, & Ruiz-Lozano, 2007; Dietz, von Bulow, Baker, & Nehls, 2011). The functions of MIP channels are regulated in various ways. "
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    Methods in enzymology 01/2015; 557:485-520. DOI:10.1016/bs.mie.2014.12.006 · 2.19 Impact Factor
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