Efficacy of vitamin A in experimentally induced acute otitis media.
ABSTRACT Vitamin A plays a role in the prevention of oxidative tissue damage. In the present study we investigated therapeutic role of this substance on healing of middle ear mucosa in experimental acute otitis media (AOM).
Otitis media was induced by inoculating Streptococcus pneumoniae via transtympanic injection. Thirty rats were divided into two groups. Group I treated with parenteral ampiciline-sulbactam. Group II received same antibiotic regimen and parenteral single dose of 100,000 IU vitamin A in palmitate form. At tenth day post-inoculation, animals were sacrificed and mucosal samples were excised from the infected tympanic cavities for histpathological examination and blood samples were obtained for measurements of activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and evaluation of levels of malondialdehyde (MDA) and nitric oxide (NO).
All the infected middle ear mucosas displayed various degrees of the inflammation, but there was no meaningful difference between two groups. However, epithelial integrity was significantly better in group II than group I (p<0.01). While serum NO and MDA levels decreased in the group receiving both antibiotic and vitamin A, serum SOD and GSH activity were found to increased. All of the statistical differences are significant.
Pretreatment with vitamin A increases antioxidant enzyme activities and reduces formation of NO and MDA. Vitamin A may be considered as an additional medicament for the medical treatment of AOM.
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ABSTRACT: Background Leukotrienes are the major factors in the formation of edema and mucus, as well as development of tuba Eustachii dysfunction in acute otitis media. We developed an experimental acute suppurative otitis media model and compared the responses of rats to penicillin and combinations of leukotriene antagonist with respect to histopathological observations conducted in early and late phases. Material and Methods A total of 83 ears from 56 Wistar rats were used in this study. Pneumococcus suspension was injected trans-tympanically into all rats. Subjects were classified into 4 different groups with 14 rats in each. In Group A, intramuscular penicillin G was injected for a period of 5 days. In Group B, intraperitoneal montelukast was injected for 21 days in addition to penicillin. In Group C, intraperitoneal montelukast isotonic NaCl in Group D was injected into rats for 21 days. Results No significant difference was found between the groups, except for mucosal vascularization with respect to mucosal and TM parameters in early phases. Furthermore, considerable deviations were observed for the recuperation of TM and mucosal inflammation for groups in which subjects were injected with montelukast as compared to other groups of the study in the late phases. Conclusions When the parameters of inflammation in the rat middle ear were compared with each other, most of these parameters did not show any statistically significant beneficial effects in montelukast and penicillin groups.Medical science monitor basic research. 01/2013; 19:246-52.
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ABSTRACT: Ear disease is a major health problem in poorly resourced countries. The role of nutritional deficiencies in its pathogenesis and in relation to chronic suppurative otitis media (CSOM) has not been reviewed previously. A systematic review was undertaken using Pubmed, SCOPUS, Cinahl on Ovid, the Cochrane Database and selected medical journals, with no language restriction. Nutritional mechanisms potentially related to ear disease and CSOM risks were reviewed. All studies (observational, case-control, cohort and clinical trials including randomised controlled trials) with nutrition-related information were included. The titles and/or abstracts of all retrieved studies were reviewed and full articles were obtained for relevant studies. Exclusion criteria were multiple publication or studies which did not report nutritional information. Supplementation studies using single micronutrients and vitamins to determine efficacy in reducing acute or chronic otitis media provided some evidence for an association of middle-ear pathology with deficiencies of zinc or vitamin A. Multi-micronutrient supplementation studies provided further support for a beneficial effect, although the number of studies was small and they were heterogeneous and uncontrolled. No human study was identified which specifically examined the association between copper, selenium or vitamin D status and middle-ear disease or infection. Particularly in developing countries, research on micronutrient status and vitamin deficiency and their influence on middle-ear disease is required to improve knowledge of the pathogenesis of middle-ear infection and to determine the relevance of nutritional interventions in prevention and treatment.Annals of Tropical Paediatrics International Child Health 07/2009; 29(2):85-99. · 0.92 Impact Factor
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ABSTRACT: Background:Leukotrienes are found to be the major factors in the formation of edema and mucus as well as development of tuba Eustachii dysfunction in acute otitis media. Montelukast sodium is a receptor antagonist of selective cystenyl leukotriene and exerts its effects through connecting to the receptor of cys leukotriene1. To develop an experimental acute suppurative otitis media model and compare the responses of rats to penicillin and combinations of leukotriene antagonist with respect to histopathological observations conducted at both early and late phases. Material and Methods: A total of eighty-three ears from fifty six Winstar rats used in this study. Pneumococcus suspension was injected transtympanically to all the rats. Subjects were classified under four different groups having 14 rats at each. In Group A, Intramusculer penicilline G was injected to for a period of five days. In Group B, intraperitoneal montelukast was injected for 21 days in addition to penicilline. In Group C, intraperitoneal montelukast, isotonic NaCl in Group D was injected to rats for 21 days. Cross-sections were semi-quantitatively graded with respect to various inflammatory components. Results: No significant difference was found between the groups, apart from mucosal vascularization with respect to mucosal and TM parameters at early phases. Furthermore, considerable deviations were observed for the recuperation of TM and mucosal inflammation for groups where subjects were injected with montekulast as compared to other groups of the study at the late phases. Conclusions: When the parameters of inflammation in the rat middle ear were compared with each other, most of these parameters did not show any statistically different beneficial effects in montelukast and penicillin groups.Medical science monitor: international medical journal of experimental and clinical research 07/2013; · 1.22 Impact Factor