Article

Preparation and PCR-amplification properties of a novel amphiphilic poly(N-vinylpyrrolidone) (PVP) copolymer.

Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Chemistry & Biodiversity (impact factor: 1.8). 03/2007; 4(2):163-74. DOI:10.1002/cbdv.200790021 pp.163-74
Source: PubMed

ABSTRACT A novel amphiphilic copolymer, P(NVP-co-TrpAMT) (9) was prepared, comprising hydrophilic N-vinylpyrrolidone (NVP; 8) and hydrophobic 'N-[(tert-butoxy)carbonyl]tryptophanamido-N'-methacryl thiourea' (TrpAMT; 7) segments. The amphiphilic copolymer 9 was characterized by (1)H-NMR, GPC-MALLS, TEM, and MTT assay. It has a critical micelle concentration (cmc) of 45.7 mg/l in aqueous solution, and good biocompatibility in vivo. According to TEM, the polymer is mostly present as spherical micelles in water, with a diameter of ca. 60-90 nm. In the presence of 0.1 mug/ml of 9, the PCR amplification of the GC-rich beta-actin was efficiently enhanced. Also, the fluorescence intensity of the reporter dye SYBR Green I was increased by 26% at the 14th cycle during real-time PCR of plasmid pUC18 DNA.

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Keywords

14th cycle
 
amphiphilic copolymer 9
 
aqueous solution
 
critical micelle concentration
 
GC-rich beta-actin
 
good biocompatibility
 
hydrophilic N-vinylpyrrolidone
 
hydrophobic 'N-[(tert-butoxy)carbonyl]tryptophanamido-N'-methacryl thiourea'
 
MTT assay
 
novel amphiphilic copolymer
 
plasmid pUC18 DNA
 
reporter dye SYBR Green
 
spherical micelles
 
TEM
 

Lifen Zhang