Pseudoruegeria aquimaris gen. nov., sp nov., isolated from seawater of the East Sea in Korea
A Gram-negative, non-motile, rod-shaped bacterial strain, SW-255(T), was isolated from seawater from Hwajinpo, on the coast of the East Sea, Korea, and subjected to a polyphasic taxonomic study. Strain SW-255(T) grew optimally at pH 7.0-8.0 and 37 degrees C in the presence of 2 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c as the major fatty acid. The DNA G+C content was 67.0 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain SW-255(T) is phylogenetically closely related to the genera Ruegeria and Silicibacter of the Alphaproteobacteria. The levels of 16S rRNA gene sequence similarity between strain SW-255(T) and the type strains of Ruegeria atlantica and two Silicibacter species were in the range 95.8-96.2 %. A phylogenetic tree based on gyrB sequences showed that strain SW-255(T) forms a distinct evolutionary lineage within the Alphaproteobacteria. Differential phenotypic properties, polar lipid profiles and DNA G+C contents, together with the phylogenetic distinctiveness, suggest that strain SW-255(T) should be distinguished from the members of the genera Ruegeria and Silicibacter. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain SW-255(T) represents a novel genus and species, for which the name Pseudoruegeria aquimaris gen. nov., sp. nov. is proposed. The type strain of Pseudoruegeria aquimaris is SW-255(T) (=KCTC 12737(T)=JCM 13603(T)).
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ABSTRACT: Two strictly aerobic, Gram-negative, rod-shaped bacteria containing photosynthesis-related genes, designated strains CL-SK44(T) and CL-JM1(T), were isolated from a culture of the marine phytoplankton Cryptomonas sp. and coastal seawater from Korea, respectively. Phylogenetic analysis of 16S rRNA gene sequences revealed that the two strains were related to members of the genera Thalassobius (95.3-96.7 % similarity), Pelagibaca (95.3-96.0 %) and Donghicola (95.6 %) in the family Rhodobacteraceae. However, the two novel strains did not form a robust clade with any species of the Roseobacter clade, forming a distinct clade. The major polar lipids of the strains were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid and an unidentified lipid, profiles that were distinguishable from those of the related genera examined. Although the level of 16S rRNA gene sequence similarity between strains CL-SK44(T) and CL-JM1(T) was very high (99.1 %), DNA-DNA relatedness between the strains was 13 %, suggesting that they represent genomically distinct species. In addition, the two strains could be differentiated based on the presence of a minor polar lipid, on the hydrolysis of gelatin and the utilization of carbon sources. Based on the data from the present study, strains CL-SK44(T) and CL-JM1(T) are considered to represent separate novel species of a new genus of the family Rhodobacteraceae, for which the names Marivita cryptomonadis gen. nov., sp. nov. (type species) and Marivita litorea sp. nov. are proposed. The type strains of Marivita cryptomonadis and Marivita litorea are CL-SK44(T) (=KCCM 90070(T)=JCM 15447(T)) and CL-JM1(T) (=KCCM 90071(T)=JCM 15446(T)), respectively.
International Journal of Systematic and Evolutionary Microbiology 07/2009; 59(Pt 7):1568-75. DOI:10.1099/ijs.0.005462-0 · 2.51 Impact Factor
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ABSTRACT: A whitish Gram-negative, motile, aerobic bacterium, designated strain H 14(T), was isolated from seawater collected at St Kilda beach in Port Phillip Bay, Melbourne, Australia. Analysis of 16S rRNA gene sequences revealed that the organism belonged to the Roseobacter lineage of the class Alphaproteobacteria, forming a distinct evolutionary lineage at the genus level. Strain H 14(T) was distantly related to the genera Nautella, Ruegeria and Pseudoruegeria (family Rhodobacteraceae). Strain H 14(T) was unable to degrade gelatin, casein, chitin, agar and starch, did not produce any carotenoids, did not possess bacteriochlorophyll a and had a limited ability to utilize carbon sources. Strain H 14(T) grew with concentrations of 1-8 % (w/v) NaCl and over a temperature range of 5-35 degrees C. Phosphatidylglycerol was the major phospholipid (90 %); phosphatidylcholine (7.9 %) and phosphatidylethanolamine (2.0 %) were present in minor quantities. The predominant fatty acids were C(18 : 1)omega7c (82.4 %), C(18 : 1)omega9c (5.1 %) and C(18 : 0) (3.8 %). The DNA G+C composition for strain H 14(T) was 59.1 mol%. Based on the results of physiological, biochemical, chemotaxonomic and phylogenetic investigations, a new genus, Celeribacter gen. nov., with the type species Celeribacter neptunius sp. nov. is proposed. The type strain of the type species is H 14(T) (=KMM 6012(T)=CIP 109922(T)).
International Journal of Systematic and Evolutionary Microbiology 09/2009; 60(Pt 7):1620-5. DOI:10.1099/ijs.0.014159-0 · 2.51 Impact Factor
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ABSTRACT: A Gram-negative-staining, non-motile and rod-shaped bacterial strain, HD-43(T), was isolated from a tidal flat sediment collected from Hwang-do, an island of Korea. Strain HD-43(T) grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HD-43(T) clustered with Pseudoruegeria aquimaris SW-255(T). It exhibited 96.6 % 16S rRNA gene sequence similarity and 79.4 % gyrB sequence similarity with P. aquimaris SW-255(T). Strain HD-43(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The DNA G+C content was 73.5 mol%. The mean DNA-DNA relatedness between strain HD-43(T) and P. aquimaris SW-255(T) was 5 %. Differential phenotypic properties demonstrated that strain HD-43(T) is clearly distinguishable from P. aquimaris. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain HD-43(T) is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria lutimaris sp. nov. is proposed. The type strain is HD-43(T) (=KCTC 22690(T) =CCUG 57754(T)).
International Journal of Systematic and Evolutionary Microbiology 09/2009; 60(Pt 5):1177-81. DOI:10.1099/ijs.0.015073-0 · 2.51 Impact Factor
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