Palmitic acid-modified poly-L-lysine for non-viral delivery of plasmid DNA to skin fibroblasts.
ABSTRACT Palmitic acid conjugates of poly-L-lysine (PLL-PA) were prepared, and their ability to deliver plasmid DNA into human skin fibroblasts was evaluated in vitro. The conjugates were capable of condensing a 4.7 kb plasmid DNA into 50-200 nm particles (mean +/- SD = 112 +/- 34 nm), which were slightly smaller than the particles formed by PLL (mean +/- SD = 126 +/- 51 nm). Both PLL and PLL-PA were readily taken up by the cells, but PLL-PA delivered the plasmid DNA into a higher proportion of cells. DNA delivery was found to be reduced by endocytosis inhibitor Brefeldin A, suggesting an active mechanism of particle uptake. Using enhanced green fluorescent protein (EGFP) as a reporter gene, PLL-PA was found to give the highest number of EGFP-positive cells among several carriers tested, including polyethyleneimine, Lipofectamine-2000, and an adenovirus. Although some carriers gave a higher percentage of EGFP-positive cells than PLL-PA, they were also associated with higher toxicities. We conclude that PLL-PA is a promising gene carrier for non-viral modification of human fibroblasts.
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ABSTRACT: Research activity in bioengineering at Canadian universities has been surveyed. Details were provided by chemical engineering departments in response to a common request for information on activities by individual researchers and for key publications. The information provided has been grouped by topics within the broad theme of "Bioengineering," and contributions from individual departments have been summarized within these topics. Although many aspects of bioengineering research are being pursued in Canada, it would appear as though environmental biotechnology, biomaterials, and tissue/cell culture are the most active areas under investigation.Biotechnology Progress 24(4):795-806. · 2.34 Impact Factor