Article

[Construction and expression of recombinant eucaryotic expression plasmid of amastin gene of Leishmania Donovani].

Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 04/2007; 23(3):217-9. pp.217-9
Source: PubMed

ABSTRACT To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani and detect expression of the gene in NIH3T3 cells.
Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid was named pcDNA3.1-amastin. NIH3T3 cell was transfected by pcDNA3.1-amastin. Transient and stable expression of amastin gene were detected by immunofluoresence and RT-PCR.
It was found that there was high green fluorescence on the cell membrane and inside the cell. It showed that NIH3T3 cell was transfected by pcDNA3.1-amastin successfully.
A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.

0 0
 · 
0 Bookmarks
 · 
30 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

amastin gene
 
eukaryotic expression vector pcDNA3.1(+)
 
Leishmania Donovani
 
NIH3T3 cell
 
NIH3T3 cells
 
nuclear DNA
 
pcDNA3.1-amastin
 
pcDNA3.1-amastin. NIH3T3 cell
 
pcDNA3.1-amastin. Transient
 
recombinant eukaryotic expression plasmid
 
recombinant plasmid
 
RT-PCR
 
stable expression