Interleukin-12- and interferon-?-mediated natural killer cell activation by Agaricus blazei Murill

Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Immunology (Impact Factor: 3.8). 07/2007; 121(2):197-206. DOI: 10.1111/j.1365-2567.2006.02558.x
Source: PubMed


Dried fruiting bodies of Agaricus blazei Murill (A. blazei) and its extracts have generally used as complementary and alternative medicines (CAMs). Here, we report that the oral administration of A. blazei augmented cytotoxicity of natural killer (NK) cells in wild-type (WT) C57BL/6, C3H/HeJ, and BALB/c mice. Augmented cytotoxicity was demonstrated by purified NK cells from treated wild-type (WT) and RAG-2-deficient mice, but not from interferon-gamma (IFN-gamma) deficient mice. NK cell activation and IFN-gamma production was also observed in vitro when dendritic cell (DC)-rich splenocytes of WT mice were coincubation with an extract of A. blazei. Both parameters were largely inhibited by neutralizing anti-interleukin-12 (IL-12) monoclonal antibody (mAb) and completely inhibited when anti-IL-12 mAb and anti-IL-18 mAb were used in combination. An aqueous extract of the hemicellulase-digested compound of A. blazei particle; (ABPC) induced IFN-gamma production more effectively, and this was completely inhibited by anti-IL-12 mAb alone. NK cell cytotoxicty was augmented with the same extracts, again in an IL-12 and IFN-gamma-dependent manner. These results clearly demonstrated that A. blazei and ABPC augmented NK cell activation through IL-12-mediated IFN-gamma production.

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    • "Immunomodulatory and therapeutic efficacy of mushroom polysaccharides have been extensively studied in various experimental models including rats (Lu et al., 2003; Li et al., 2008), mice (Takimoto et al., 2004; Yuminamochi et al., 2007) and rabbits (Ikuzawa et al., 1988) with promising results; although a very limited studies in this regard has been conducted in avian birds (Guo et al., 2004). Keeping in view, the present study reports the immunomodulatory activity of Pleurotus ostreatus and Pleurotus sajor-caju derived polysaccharides and its subsequent therapeutic efficacy against Eimeria infection in chicken. "
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    • "In Japan, some mushrooms are widely used as a health food, thereby expecting pharmacological activity [24], [33], [34]. The fruiting body of an edible Basidiomycetes mushroom, A. brasiliensis, rich in β-glucan induces NK cell activation through an IFN-γ-dependent pathway [23], [35]. Moreover, research has revealed that GM-CSF is required for the immunoenhancing activities induced by fungal-derived β-glucans [36]–[38], and that this mushroom has polyphenol-related enzymes such as polyphenol oxidase, and peroxidase [39], [40]. "
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    PLoS ONE 04/2012; 7(4):e36025. DOI:10.1371/journal.pone.0036025 · 3.23 Impact Factor
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    • "Some complexes from mushrooms are able to stimulate the non-specific immune system through the stimulation of the host's defence mechanism [2–16]. The immunomodulatory actions associated with mushrooms intake have been suggested as being due to a number of isolated fractions from mushrooms, including the β-d-glucans and other polysaccharides [17] [18] [19] "
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