Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells
ABSTRACT Oxidative stress plays an important role in Alzheimer's disease and other neurodegenerative disorders. Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L, shows potent antioxidant property. In this paper, the neuroprotective effects of salidroside on hydrogen peroxide (H2O2)-induced apoptosis in SH-SY5Y cells were investigated. Pretreatment with salidroside markedly attenuated H2O2-induced cell viability loss and apoptotic cell death in a dose-dependent manner. The mechanisms by which salidroside protected neuron cells from oxidative stress included the induction of several antioxidant enzymes, thioredoxin, heme oxygenase-1, and peroxiredoxin-I; the downregulation of pro-apoptotic gene Bax and the upregulation of anti-apoptotic genes Bcl-2 and Bcl-X(L). Furthermore, salidroside dose-dependently restored H2O2-induced loss of mitochondrial membrane potential as well as the elevation of intracellular calcium level. These results suggest that salidroside has protective effects against oxidative stress-induced cell apoptosis, which might be a potential therapeutic agent for treating or preventing neurodegenerative diseases implicated with oxidative stress.
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09/2015; 3. DOI:10.7831/ras.3.40
- "200 µM 24 h ~70% Nakano et al. (2011) 200 µM 24 h ~60% Xiao et al. (2013) 200 µM 24 h ~50% Zhang et al. (2007) 100 µM 24 h ~65% Zhang et al. (2007) 100 µM 24 h ~40% Suematsu et al. (2011) Please cite this article as: "
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- "Salidroside (p-hydroxyphenethyl-b-D-glucoside) is the main active ingredient from Rhodiola rosea L, and has displayed many pharmacological properties including anti-aging, anti-fatigue, anti-oxidant, anti-cancer and anti-inflammation effects     . For example, salidroside inhibits hydrogen peroxide (H 2 O 2 )-induced damage of SH-SY5Y human neuroblastoma cells and rat hippocampal neurons  . Meanwhile, salidroside protects cells from glutamate toxicity, as well as calcium-overload and cobalt chloride stresses [9,12]. "
ABSTRACT: Oxygen glucose deprivation (OGD)/re-oxygenation has been applied to cultured cardiomyocytes to create a cellular model of ischemic heart damage. In the current study, we explored the potential role of salidroside against OGD/re-oxygenation-induced damage in H9c2 cardiomyocytes, and studied the underlying mechanisms. We found that OGD/re-oxygenation primarily induced necrosis in H9c2 cells, which was inhibited by salidroside. Salidroside suppressed OGD/re-oxygenation-induced reactive oxygen species (ROS) production, p53 mitochondrial translocation and cyclophilinD (Cyp-D) association as well as mitochondrial membrane potential (MMP) decrease in H9c2 cells. Meanwhile, salidroside activated Akt and promoted transcription of NF-E2-related factor 2 (Nrf2)-regulated genes (heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO-1)). Significantly, Nrf2 shRNA knockdown or Akt inhibitors (LY 294002 and Wortmannin) not only prevented salidroside-induced HO-1/NQO-1 transcription, but also alleviated salidroside-mediated cytoprotective effect against OGD/re-oxygenation in H9c2 cells. These observations suggest that salidroside activates Nrf2-regulated anti-oxidant signaling, and protects against OGD/re-oxygenation-induced H9c2 cell necrosis via activation of Akt signaling.Biochemical and Biophysical Research Communications 07/2014; 451(1). DOI:10.1016/j.bbrc.2014.07.072 · 2.30 Impact Factor
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- "Salidroside and tyrosol, rosin, rosavin, rosarin, cinnamyl alcohol, herbacytrin, umbeliferon, esculetin, luteolin, tricetin, epigallocatechin, epigallocatechin gallate, lotaustralin, rodiocyanoside A, tannins, daucosterol and b-sitosterol, hydroxycinnamic, gallic acid, chlorogenic acid Kang et al. (1992), Krasnov et al. (1978), Krajewska- Patan et al. (2006), Wiedenfeld et al. (2007b), Zuo et al. (2007), Wong et al. (2008), Krajewska-Patan et al. (2009), Krajewska-Patan et al. (2013) R. quadrifida Salidroside and tyrosol, rosin, rosavin, rosarin, cinnamyl alcohol, rhodiooctanoside, rhodiolin, mongrhoside, rhodiocyanosides A and B, rhodioflavonoside, rhodiooctanoside, tricetin, L-rhamnopyranoside, osmaronin, chlorogenic acid Yoshikawa et al. (1996), Altantsetseg et al. (2007), Wiedenfeld et al. (2007a) R. rosea (in total over 140 compounds) salidroside and tyrosol, rosin, rosavin, rosarin, cinnamyl alcohol, epigallocatechin, epigallocatechin gallate, lotaustralin, rodiocyanoside A, herbacetin, kaemferol, rosiridol and rosaridin, daucosterol and b-sitosterol, Rhodiolosid A-C, organic acids, tannins, waxes, fats, proanthocyanidins, sachaliside, gallic, hydroxycinnamic acid, acetylrodalgin and tricin Saratikov et al. (1967), Kurkin et al. (1985, 1986), Kurkin and Zapesochnaya (1986), Akgul et al. (2004), Yousef et al. (2006), Altantsetseg et al. (2007), Ali et al. (2008), Ma et al. (2013) R. sachalinensis (in total over 44 compounds) salidroside and tyrosol, rosarin, rosavin, cinnamyl alcohol, multiflorin B, tricetin, afzelin, kaempferol, rhodionin, rhodiosin, gallic acid, sachalosides I –V, sacranoside A, rhodiocyanoside A, lotaustralin, hetorodendrin, -glucopyranoside Lee et al. (2000), Nakamura et al. (2007), Zhang et al. (2007a), Choe et al. (2012) Phytochem Rev included in the list of endangered plant species in many countries (Russia, Great Britain, the Czech Republic, Bosnia and Herzegovina, vulnerable in Slovakia; in Bulgaria collection is strictly forbidden; in Poland R. rosea is only found in National Parks) (Galambosi 2006; Kołodziej and Sugier 2012). Sufficient yields of field-cultivated R. rosea roots/rhizomes could be obtained within 5–7 years of cultivation, however the costs are high (Galambosi 2006; Peschel et al. 2013). "
ABSTRACT: Rhodiola (Crassulaceae) an arctic-alpine plant, is extensively used in traditional folk medicine in Asian and European countries. A number of investigations have demonstrated that Rhodiola prep-arations exhibit adaptogenic, neuroprotective, anti-tumour, cardioprotective, and anti-depressant effects. The main compounds responsible for these activities are believed to be salidroside, rosin and its derivatives which became the target of biotechnological investi-gations. This review summarizes the results of the diverse biotechnological approaches undertaken to enhance the production of salidroside, rosin and its derivatives in callus, cell suspension and organ in vitro cultures of selected Rhodiola species. TyrDC Tyrosine decarboxylase UDP UDP-glucose:tyrosol glucosyltransferase UGT Uridine diphosphate dependent glucosyltransferase IntroductionPhytochemistry Reviews 06/2014; 14(4). DOI:10.1007/s11101-014-9368-y · 2.41 Impact Factor