Flow cytometric analysis of CD4+ T cell receptor zeta chain deficiency in patients with systemic lupus erythematosus.

Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poland.
Advances in Medical Sciences (Impact Factor: 1.11). 02/2006; 51:181-3.
Source: PubMed


It has been reported that a dysfunction of T lymphocytes can be responsible for alteration in immune system in patients with systemic lupus erythematosus (SLE).
Using flow cytometric analysis, we determined the abnormalities of T cell receptor zeta (TCR zeta) chain contents in CD4+ T cells of SLE patients.
We observed a decrease in mean fluorescence intensity of TCR zeta in CD4+ T cells of patients with SLE. The multiple analysis did not show a correlation between gender, age, disease specific manifestation, treatment, duration and TCR zeta mean fluorescence intensity in CD4+ T cells.
High prevalence of TCR zeta chain deficiency in CD4+ T cells confirms the significance of this signaling molecule in SLE pathogenesis.

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    ABSTRACT: T cells from patients with systemic lupus erythematosus (SLE) exhibit several discrete and specific defects that alter signaling pathways and, thus, the gene expression pattern and behavior upon stimulation. Rewiring of the CD3 complex and aggregation of surface-membrane lipid rafts grant SLE T cells a lower activation threshold and distort the ensuing signaling events. Additionally, increased expression of adhesion molecules within aggregated lipid rafts guides them to target organs. Aberrant cell signaling causes altered transcription factor expression and abnormal DNA-methylation patterns that lead to skewed gene expression. The result is an abnormally functioning T cell that exhibits several molecular alterations that can be exploited as therapeutic or diagnostic markers.
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