Influence of semicrystalline order on the second-harmonic generation efficiency in the anisotropic bands of myocytes

University of Toronto, Toronto, Ontario, Canada
Applied Optics (Impact Factor: 1.78). 05/2007; 46(10):1852-9. DOI: 10.1364/AO.46.001852
Source: PubMed


The influence of semicrystalline order on the second-harmonic generation (SHG) efficiency in the anisotropic bands of Drosophila melanogaster sarcomeres from larval and adult muscle has been investigated. Differences in the semicrystalline order were obtained by using wild-type and mutant strains containing different amounts of headless myosin. The reduction in semicrystalline order without altering the chemical composition of myofibrils was achieved by observing highly stretched sarcomeres and by inducing a loss of viability in myocytes. In all cases the reduction of semicrystalline order in anisotropic bands of myocytes resulted in a substantial decrease in SHG. Second-harmonic imaging during periodic contractions of myocytes revealed higher intensities when sarcomeres were in the relaxed state compared with the contracted state. This study demonstrates that an ordered semicrystalline arrangement of anisotropic bands plays a determining role in the efficiency of SHG in myocytes.

Download full-text


Available from: Richard Cisek,
  • Source
    • "SB sarcomeric SHG pattern has been observed in gastrocnemius of adult xenopus (Tiaho et al., 2007; Recher et al., 2009), frog tibialis anterior muscle (Vanzi et al., 2006), mouse quadriceps or gastrocnemius muscles (Legare et al., 2007; Plotnikov et al., 2008), mouse and human hind lamb muscles (Ralston et al., 2008), veal cutlet muscles (Odin et al., 2008), nematode body-wall muscles (Psilodimitrakopoulos et al., 2009b). By contrast, DB sarcomeric SHG pattern has been observed in Caenorhabditis elegans body-wall muscles (Campagnola et al., 2002; Mohler et al., 2003), frog heart muscles (Boulesteix et al., 2004), mouse tibialis anterior muscle (Both et al., 2004), mouse leg and chicken heart (Plotnikov et al., 2006), rat temporalis muscle (Psilodimitrakopoulos et al., 2009a), rabbit psoas muscles (Vanzi et al., 2006) and drosophila flight muscles (Greenhalgh et al., 2007; Prent et al., 2008). One can notice that in nematod body-wall muscles both patterns have been reported using high optical resolution objectives. "
    [Show abstract] [Hide abstract]
    ABSTRACT: To understand the reported difference between double band, sarcomeric second harmonic generation pattern of isolated myofibril and predominant single band pattern found in thick muscle tissues, we studied the effect of myofibril preparation on the second harmonic generation pattern. We found that double band sarcomeric second harmonic generation pattern usually observed in myofibrils (prepared from fresh tissue) is due to muscle alteration during the mixing and triton treatment processes. Single band sarcomeric second harmonic generation pattern could be observed in isolated myofibrils when this alteration is previously prevented using paraformaldehyd fixed tissue. We conclude that single band sarcomeric second harmonic generation pattern is a signature of adult muscle myofibrils in normal physiological condition, suggesting that sarcomeric second harmonic generation patterns could be used as a valuable diagnosis tool of muscle health.
    Journal of Microscopy 08/2010; 241(2):207-11. DOI:10.1111/j.1365-2818.2010.03425.x · 2.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Real time multiphoton excitation fluorescence microscopy of enhanced yellow fluorescent protein was performed with low photobleaching using a femtosecond Yb:KGd(WO4)2 laser. Using a conventional Ti:S laser this was previously unfeasible due to low excitation efficiency.
    Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on; 01/2009
  • Source

Show more