Effect of preservation solution on graft viability in single-lung transplantation from heart-beating donors in pigs
ABSTRACT Low-potassium-dextran preservation solution Perfadex (PER) may provide better outcome of transplanted lungs than high-potassium Euro-Collins (EC) solution. However, there are no comparative studies of the recipient inflammatory response to the graft.
The purpose of this study was to compare EC versus PER as preservation solutions with respect to the functional performance and inflammatory response in single-lung transplantation from heart-beating donors in pigs.
The donor left lung flushed with the corresponding cold preservation solution was stored at 3 degrees C for 3 hours. We assessed hemodynamic values and pulmonary function in the recipient over a 2-hour reperfusion period calculated as percent of basal values, and expressed as mean of the reperfusion period. Interleukin-8 (IL-8) concentration in the donor was estimated in bronchoalveolar lavage fluid 2 hours after recipient reperfusion. Biopsies of the donor right lung and the transplanted lung were obtained to measure myeloperoxidase (MPO) activity. IL-8 and MPO values were expressed as percent of the donor value. We evaluated the wet/dry pulmonary weight ratio (W/D), polymorphonuclear neutrophil count (PMN), and a score of histological damage in the transplanted graft.
Pulmonary function evaluated by % static: 66.6 +/- 6.8 (EC), 82.3 +/- 10.2 (PER), and dynamic: 74.0 +/- 7.3 (EC), 89.3 +/- 7.7 (PER) compliances, as well as % IL-8: 562.5 +/- 168.6 (EC), 232.3 +/- 148.7 (PER), % MPO: 485.9 +/- 194.9 (EC), 140.8 +/- 21.1 (PER), W/D: 9.9 +/- 3.1 (EC), 6.8 +/- 1.4 (PER), PMN 13.5 +/- 6.8 (EC), 5.5 +/- 3.3 (PER) and the histological damage score: 3.0 +/- 1.5 (EC), 0.7 +/- 0.4 (PER) showed significant differences between the EC and the PER (P < .01).
PER affords good lung preservation with early graft function and modest evidences of inflammation, lung injury, and edema compared with the EC perfused lung.
SourceAvailable from: Cristiano Feijó Andrade[Show abstract] [Hide abstract]
ABSTRACT: Objective: To evaluate the effect of lung ischemic preconditioning (IPC) on normothermic ischemia/reperfusion (I/R) injury in a rat model, quantifying the production of reactive oxygen species. Methods: Forty-seven male Wistar rats were randomized into four groups: control, sham, I/R and IPC. Control group animals were anesthetized and killed by decapitation, after which pneumonectomy was performed and the left lungs were stored in liquid nitrogen. Sham, IPC and I/R group rats were anesthetized, tracheostomized, ventilated, anticoagulated and submitted to left thoracotomy with dissection of the left pulmonary artery for clamping. Sham group rats underwent dissection of the left pulmonary artery, I/R group rats underwent 30 min of total hilar clamping, and IPC group rats underwent 5-min clamping of the left pulmonary artery followed by 30 min of total hilar clamping. Lungs were reperfused for 90 min and ventilated with the same parameters, with additional positive end-expiratory pressure of 1 cmH 2 O. Hemodynamic and blood gas values were obtained prior to thoracotomy, prior to total hilar clamping, after 30 min of reperfusion and after 90 min of reperfusion. Lipid peroxidation was determined by measuring levels of thiobarbituric acid reactive substances. Results: There were no significant differences among the groups in terms of the levels of thiobarbituric acid reactive substances. Nor were there any significant differences among the sham, I/R and IPC groups in terms of arterial oxygen tension, arterial carbon dioxide tension or hemodynamic values. Conclusions: In an in situ I/R rat model, 5-min IPC of the left pulmonary artery does not attenuate I/R injury.
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ABSTRACT: Purpose of review: The imbalance between patients requiring lung transplantation and the availability of suitable donor organs is one of the major factors contributing to long waiting times and considerable mortality on the waiting list. The lung is an especially vulnerable organ, and particularly prone to infection, due to its direct contact with the environment. This situation further diminishes the potential number of transplantable organs. In this situation, the use of lungs from non-heart-beating donors represents an approach to overcome this limitation. A broad application of this method could help to significantly expand the currently available donor-organ pool. Recent findings: This paper aims to outline the problems related to the use of lungs from non-heart-beating donors, to give an overview on recent experimental advancements, and to review the current clinical status of the method. Furthermore, an outlook on its potential future applications is given. Summary: The use of non-heart-beating lung donors has become routine in some centers, leading to an expansion of the potential donor pool. More long-term outcome data are required to verify the equality or superiority of non-heart-beating donor lungs compared with heart-beating, brain-dead donors. Non-heart-beating donation can be expected to gain a far more widespread use in the future.Current Opinion in Organ Transplantation 09/2007; 12(5):459-463. DOI:10.1097/MOT.0b013e3282eff56d · 2.38 Impact Factor
Jornal Brasileiro de Pneumologia 01/2008; 34(8). DOI:10.1590/S1806-37132008000800007 · 1.27 Impact Factor