Article

Effect of preservation solution on graft viability in single-lung transplantation from heart-beating donors in pigs.

Institute of Cardiology and Cardiovascular Surgery of the Favaloro Foundation, Buenos Aires, Argentina.
Transplantation Proceedings (impact factor: 1). 04/2007; 39(2):355-7. DOI:10.1016/j.transproceed.2007.01.014 pp.355-7
Source: PubMed

ABSTRACT Low-potassium-dextran preservation solution Perfadex (PER) may provide better outcome of transplanted lungs than high-potassium Euro-Collins (EC) solution. However, there are no comparative studies of the recipient inflammatory response to the graft.
The purpose of this study was to compare EC versus PER as preservation solutions with respect to the functional performance and inflammatory response in single-lung transplantation from heart-beating donors in pigs.
The donor left lung flushed with the corresponding cold preservation solution was stored at 3 degrees C for 3 hours. We assessed hemodynamic values and pulmonary function in the recipient over a 2-hour reperfusion period calculated as percent of basal values, and expressed as mean of the reperfusion period. Interleukin-8 (IL-8) concentration in the donor was estimated in bronchoalveolar lavage fluid 2 hours after recipient reperfusion. Biopsies of the donor right lung and the transplanted lung were obtained to measure myeloperoxidase (MPO) activity. IL-8 and MPO values were expressed as percent of the donor value. We evaluated the wet/dry pulmonary weight ratio (W/D), polymorphonuclear neutrophil count (PMN), and a score of histological damage in the transplanted graft.
Pulmonary function evaluated by % static: 66.6 +/- 6.8 (EC), 82.3 +/- 10.2 (PER), and dynamic: 74.0 +/- 7.3 (EC), 89.3 +/- 7.7 (PER) compliances, as well as % IL-8: 562.5 +/- 168.6 (EC), 232.3 +/- 148.7 (PER), % MPO: 485.9 +/- 194.9 (EC), 140.8 +/- 21.1 (PER), W/D: 9.9 +/- 3.1 (EC), 6.8 +/- 1.4 (PER), PMN 13.5 +/- 6.8 (EC), 5.5 +/- 3.3 (PER) and the histological damage score: 3.0 +/- 1.5 (EC), 0.7 +/- 0.4 (PER) showed significant differences between the EC and the PER (P < .01).
PER affords good lung preservation with early graft function and modest evidences of inflammation, lung injury, and edema compared with the EC perfused lung.

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    Article: Ischemic preconditioning by selective occlusion of the pulmonary artery in rats.
    Eduardo Sperb Pilla, Giovani Schirmer Vendrame, Pablo Gerardo Sánchez, Gustavo Grun, Eduardo Fontena, Luiz Alberto Forgiarini, Norma Anair Possa Marroni, Cristiano Feijó Andrade, Paulo Francisco Guerreiro Cardoso
    [show abstract] [hide abstract]
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Keywords

% MPO
 
% static
 
3 hours
 
affords good lung preservation
 
basal values
 
bronchoalveolar lavage fluid 2 hours
 
corresponding cold preservation solution
 
donor value
 
EC perfused lung
 
functional performance
 
graft function
 
hemodynamic values
 
high-potassium Euro-Collins
 
Low-potassium-dextran preservation solution Perfadex
 
MPO values
 
pulmonary function
 
recipient reperfusion
 
transplanted graft
 
transplanted lung
 
wet/dry pulmonary weight ratio