Article

Modelling the application of adaptive optics to wide-field microscope live imaging

Weizmann Institute of Science, Rehovot 76100, Israel.
Journal of Microscopy (Impact Factor: 2.15). 05/2007; 226(Pt 1):33-42. DOI: 10.1111/j.1365-2818.2007.01751.x
Source: PubMed

ABSTRACT Wide-field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often significantly degraded due to aberrations that occur under normal imaging conditions. In this article, we examine the use of adaptive optics technology to dynamically correct these problems to achieve close to ideal diffraction limited performance. Simultaneously, this technology also allows ultra-rapid focusing without having to move either the stage or the objective lens. We perform optical simulations to demonstrate the degree of correction that can be achieved.

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    • "corrected for a single depth. Researchers have proposed numerous ways to reduce aberrations, including introducing additional optics (akin to the cover slip corrector found on some objectives), changing the index of refraction of the immersion medium, or employing computer-controlled deformable mirrors (Albert et al., 2000; Booth et al., 2002; Sherman et al., 2002; Kam et al., 2007; Wright, 2007). However, these techniques offer only a limited ability to correct for aberrations that vary spatially. "
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