Porcine circovirus type 2 and associated diseases in Romania — Short communication

Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Manastur 3-5, Romania.
Acta Veterinaria Hungarica (Impact Factor: 0.65). 04/2007; 55(1):151-6. DOI: 10.1556/AVet.55.2007.1.14
Source: PubMed


Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). This report describes the first detection of PCV2 and associated diseases in a Romanian swine herd located in Transylvania. The clinical signs, pathological and histopathological changes observed in affected pigs were similar to those previously described for PDNS and PMWS. Polymerase chain reaction and in situ hybridisation were used for the detection of PCV2 nucleic acids from tissues and serum samples. Complete PCV2 genomes of both PMWS and PDNS cases were sequenced and analysed, and by comparing them with each other no genomic differences could be detected. The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary.

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Available from: Dániel Cadar, Sep 26, 2014
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    • "Two years later the first official data on detection of PCV-2 in PMWS-affected pigs at antigenic and genetic level was described in Slovakia (Pistl et al., 2009). In the last decade, several studies were published, which document PCV-2 infection and the occurrence of PMWS in domestic or feral pigs in Poland (Stadejek et al., 2006), Hungary (Kiss et al., 2000; Tibor, 2004), Romania (Cadar et al., 2007), Slovenia (Toplak et al., 2004), Croatia (Lipej et al., 2005), Greece (Sofia et al., 2008), Czech Republic (Celer and Carasova, 2002; Sedlak et al., 2008) and Austria (Schmoll et al., 2002, 2003). Till to date, no data is available that presents the prevalence of PCV-2 infection in the pig population in Slovakia. "
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    ABSTRACT: The prevalence of porcine circovirus 2 (PCV-2) infection in the pig population in Slovakia was investigated. Sera from pigs suspected for post-weaning multisystemic wasting syndrome (PMWS) as well as clinically healthy pigs were tested for viral DNA and specific IgM and IgG antibodies. Pigs (n = 198) were categorized to weaning, grower and fattening ones and sows. The results showed that PCV-2 antibodies were present in 53.4% of PMWS-suspects, in 50.0% of healthy pigs and in 69.0% of sows. In PMWS-suspect grower pigs, 40.7% were positive for IgM+IgG antibodies and 22.2% for viral DNA. In PMWS-suspect fattening pigs, 50.0% were positive for IgM+IgG antibodies and 25.0% for viral DNA. In healthy fattening pigs, almost 90.0% were positive for IgG antibodies and 38.5% for viral DNA. The highest proportion of PMWS-suspects was in grower pigs and specific antibodies were increasing with the age of pigs. A combination of positivities for IgG+IgM antibodies and viral DNA was a highly significant marker of PMWS. Viral DNA was detected in seropositive as well as seronegative PMWS-suspects. Overall, in all categories of pigs tested, specific antibodies and viral DNA were detected in 54.0% and 35.5%, respectively.
    Acta virologica 01/2011; 55(3):267-71. DOI:10.4149/av_2011_03_267 · 1.28 Impact Factor
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    ABSTRACT: The present paper was meaned to be a serological, biomolecular and histopathological screening about the presence of porcine circovirus of type 2, in three swine farms from Romania, as a component part of some complex investigations regarding the opportunity of conceiving and introducing in the immunoprofilaxis programs from our country of an efficient vaccine against porcine circovirosis. By immunoenzymatic processing 111 serum samples, harvested from piglets, sows, boars, swine in fattening, it has been established that at each of the three farms that was under study, over 60% of the results were positive towards porcine circovirus of type 2 (PCV2). The presence of PCV2 in the respective farms demonstrated it and the specific amplicons obtained, at the Pasteur Institute from Bucharest, as well as at the Institute of Veterinary Medicine from Budapest, after processing by PCR of some extracts from organs samples harvested from dead bodies and/ or abortions. On histological preparations performed from samples with positive PCR results were observed some aspects characteristic for PCV2 infection (lymphocytes depletion at the level of lymphoid organs, respectively lymphohystiocytic bronchopneumonia), aspects which, correlated with the results of the other investigations, confirmed the evolution of circovirosis in swine herds under study. Considering the important economical losses that swine circovirosis can produce directly and/ or indirectly, as well as the alarming results obtained by this study, we consider that it is necessary the achievement of this kind of screening at a larger scale and reconsidering the importance of the immune prophylaxis specific for this disease.
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    ABSTRACT: Definition of circuit of PCV2 on flow technology in a swine herd requires an epidemiological investigation of PCV2 infection in all swine categories (from sows to fattening pigs) in a farm affected by PCV2 associated diseases. To achieve this goal the swine herds were evaluated for the presence of PCV2 antibodies using an ELISA and indirect-fluorescent antibody procedure following the kinetics of PCV2 antibodies to subclinical infected (PCV2 positive) animals and PMWS/PDNS affected pigs. Clinical expression of porcine circovirus 2 (PCV2) infections in swine may result in several distinct syndromes and diseases including post-weaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS) and other diseases. The first description of presence of PCV2 and associated diseases in Romania were described in 2003 (1). PCV2 antibodies have been found in pigs from all continents, usually with very high seroprevalence (4). The aim of this study was to detect the presence of PCV2 specific G and M immunoglobulins in sera of subclinical infected and PMWS/PDNS affected pigs. We performed the titration of specific PCV2 IgG antibodies to evaluate the kinetics of antibodies on the technology lines of the farms, to all swine categories taken into study. Therefore, it may find data on time of seroconversion, which of the categories of swine are exposed to infection following the correlation between the titer of antibodies and time of PCV2 infection, as identify the critical period of exposure to PCV2 infection and time of occurrence and manifestation of PCV2 associated diseases (PMWS and PDNS).
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