Identification of the distinct promoters for the two transcripts of apoptosis related protein 3 and their transcriptional regulation by NFAT and NFκB
ABSTRACT APR3 (apoptosis related protein 3) is a novel gene highly conserved across species. Analysis of the data about APR3 available at GEO profiles revealed consistent and significant changes of APR3 expression level in certain developmental and inflammatory processes. Based on the search and analysis of all the submitted mRNA sequence, we postulated that the two transcripts may arise from separate promoter activities rather than previously assumed alternative splicing. Through reporter assay and PCR data, we identified the distinct promoters for the two transcripts of APR3. Furthermore, exogenous expression of a constitutively active mutant of transcription factor NFAT was able to enhance both the promoter activities of APR3. Sequential deletion of the promoter from the 5' side and mutation of the promoter suggested the functional NFAT binding sites might localize between -96 bp and -47 bp. In contrast, exogenous expression of a constitutively active mutant of the transcription factor NFkB inhibited APR3 transcription. Our data suggested that APR3 might be functionally important in certain processes under which NFAT and/or NFkappaB are/is activated.
Conference Paper: Ambipolar field-effect transistors based on fullerene peapods[Show abstract] [Hide abstract]
ABSTRACT: The electrical transport properties of C<sub>70</sub> and C<sub>60</sub> fullerene peapods are investigated. We report the fabrications and performances of field-effect transistors (FETs) based on C<sub>70</sub> and C<sub>60</sub> fullerene peapods. Most of the fullerene peapod-FETs exhibit ambipolar characteristics at room temperature in air. The origin of ambipolar behavior is also qualitatively discussed. The ambipolar FETs based on fullerene peapods exhibit hysteresis effect in their electrical characteristics.Solid-State and Integrated Circuits Technology, 2004. Proceedings. 7th International Conference on; 11/2004
- Molecular and Cellular Biochemistry 10/2007; 306(1-2). DOI:10.1007/s11010-007-9572-9 · 2.39 Impact Factor
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ABSTRACT: RNA interference is an appealing and promising therapeutic approach in cancer and other diseases. Designing novel strategies aiming to increase the efficiency, duration, and reduce the off-target silencing by sense strand is of great significance for its future application clinically. Here, we report that RNA duplex with the sense strand pre-cleaved at the base between base 10 and 11 relative to the 5' end of the antisense strand induced a target-specific RNA silencing effectively. Furthermore, different from the canonical RNA duplex, this novel RNA duplex rarely inhibits the luciferase activity in the reporter, bearing the target sequence corresponding to the sense strand, suggesting a less off-target effects of this novel strategy. Furthermore, the immune response of the novel RNA duplex induced a much milder immune response as seen from the NFkappaB activity. In addition, our newly designed RNA duplex should be easier for preservation than the asymmetric RNA duplex. Our results establish a novel method to design a new class of RNA duplex for improved RNA interference.Applied Microbiology and Biotechnology 12/2010; 90(2):583-9. DOI:10.1007/s00253-010-3065-6 · 3.81 Impact Factor