Bezold G, Politch JA, Kiviat NB, Kuypers JM, Wolff H, Anderson DJ. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia

Department of Obstetrics and Gynecology, Boston University, Boston, Massachusetts, United States
Fertility and sterility (Impact Factor: 4.59). 06/2007; 87(5):1087-97. DOI: 10.1016/j.fertnstert.2006.08.109
Source: PubMed

ABSTRACT To determine the prevalence of pathogens that cause sexually transmitted infections (STIs) in semen from asymptomatic male infertility patients with and without leukocytospermia (LCS), and associations between STIs, inflammatory markers, and other semen variables.
Retrospective, controlled study.
Academic Medical Center.
Two hundred and forty-one male infertility patients undergoing routine semen analysis: 132 with LCS, and 109 without LCS.
The DNA from STI pathogens (human papillomavirus [HPV], cytomegalovirus [CMV], herpes simplex virus [HSV], human herpesvirus type 6 [HHV-6], Epstein-Barr virus [EBV], hepatitis B virus [HBV], and Chlamydia trachomatis [CT]), routine semen parameters, and markers of accessory gland and epididymal function and inflammation.
The DNA from STI pathogens was detected in 45/241 (18.7%) of the samples (CMV, 8.7%; HPV, 4.5%; HHV-6, 3.7%; HSV, 3.7%; CT, 2.5%; EBV, 0.4%; and HBV, 0%), with no difference in prevalence between the LCS and non-LCS groups. The DNA of STI pathogens in semen was associated with a decrease in sperm concentration, motile sperm concentration, total sperm count, and neutral alpha-glucosidase concentration, whereas LCS was associated with a decrease in total sperm count, percent normal forms, and fructose concentration.
The DNA of STI pathogens was detected in semen from a high percentage of asymptomatic male infertility patients, and was associated with poor semen quality. Efforts to diagnose and treat subclinical genital-tract infections should be intensified.

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Available from: Nancy B Kiviat, Feb 11, 2015
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    • "According to recent findings 15–20% of infertile male subjects are affected by semen infection [8], and most data have been concordant with respect to the relevance of STDs to male infertility [7], [8]. Several STDs in semen were associated with poor sperm quality [9] and decreased sperm concentration and motility [7]. However, there are few studies evaluating these aspects, and additional epidemiological studies in different populations and clinical scenarios are needed to determine the real impact of STD pathogens on male infertility. "
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    ABSTRACT: Sexually transmitted diseases (STDs) may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR) assay for the simultaneous detection of the following STD pathogens in semen: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Herpes virus simplex (HSV) -1 and -2, and Treponema pallidum; We also investigated the potential usefulness of this M-PCR assay in screening programs for semen pathogens. In addition, we aimed: to detect human Papillomavirus (HPV) and genotypes by single PCR (sPCR) in the same semen samples; to determine the prevalence of the seven STDs, HPV and co-infections; to assess the possibility that these infections affect semen parameters and thus fertility. The overall validation parameters of M-PCR were extremely high including agreement (99.2%), sensitivity (100.00%), specificity (99.70%), positive (96.40%) and negative predictive values (100.00%) and accuracy (99.80%). The prevalence of STDs was very high (55.3%). Furthermore, associations were observed between STDs and changes in semen parameters, highlighting the importance of STD detection in semen. Thus, this M-PCR assay has great potential for application in semen screening programs for pathogens in infertility and STD clinics and in sperm banks.
    PLoS ONE 06/2014; 9(6):e98862. DOI:10.1371/journal.pone.0098862 · 3.23 Impact Factor
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    • "The relationship between HPV infection in men and abnormal sperm quality is controversial. As long as routine sperm washing fails to eliminate HPV DNA from the ejaculate, it is of crucial importance to determine whether HPV DNA sperm infection affects the quality of sperm parameters or not [16, 35]. The aim of this study was to establish the type-specific prevalence of HPV DNA infection of external genitalia and semen with at least one of the 37 most important alpha HPV types in a group of men from Slovenian infertile couples. "
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    ABSTRACT: Human papillomaviruses (HPV) are the most frequently sexually transmitted viruses and etiological agents of several human cancers. Controversial results of the role of HPV in infertile population on sperm parameters have been published. The aim of this study was to estimate the type-specific prevalence of HPV DNA infection of the external genitalia and semen in 340 Slovenian men from infertile couples and to establish the relationship between seminal HPV DNA infection and abnormal sperm parameters. Self-taken swabs of the entire penile surface and semen samples were collected, and HPV detection and genotyping were performed. HPV DNA was detected in 37.12% of external genitalia and in 13.61% of semen samples with high HPV type concordance of both sampling sites. The most prevalent HPV types in the male external genitalia were HPV-CP6108 and HPV-84. The most prevalent HPV types in semen were HPV-53 and HPV-CP6108. The prevalence of HPV infection between normozoospermic men and men with abnormal sperm parameters did not differ significantly. Sperm quality did not differ significantly between men with seminal HPV infection and uninfected men. In conclusion, the men from infertile couples are equally susceptible to HPV infection regardless of their fertile potential; seminal HPV infection does not impair sperm quality.
    04/2014; 2014(9):956901. DOI:10.1155/2014/956901
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    • "A) 4.4% (n = 45) A) HIV negative B) HIV-positive homosexual men USA, Japan B) 87.7% (n = 73) Bezold et al. 2001 [11] 3.2% (n = 252) 0% (n = 252) 7.1% (n = 252) 0% (n = 252) FCA Germany Bezold et al. 2007 [12] 3.7% (n = 241) 0.4% (n = 240) FCA Massachusetts Bocharova et al. 2007 [13] "
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    ABSTRACT: Most of the human herpesviruses can be found in semen, although the reported prevalence varies considerably between individual studies. The frequent presence of herpesvirus in semen raises the question whether sexual transmission of the virus could have an impact on human reproduction. Only few studies have associated seminal shedding of herpesviruses with impaired sperm quality, reduced fertility, or reduced chances of pregnancy, whereas most studies fail to find an association. Taken together, no firm evidence is so far linking the presence of herpesviruses in semen to impaired human reproduction.
    Virology Journal 07/2013; 10(1):226. DOI:10.1186/1743-422X-10-226 · 2.18 Impact Factor
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