Microsensor Arrays for Saliva Diagnostics

Department of Periodontology and Oral Biology, Boston University, Boston, Massachusetts, United States
Annals of the New York Academy of Sciences (Impact Factor: 4.38). 04/2007; 1098(1):389-400. DOI: 10.1196/annals.1384.031
Source: PubMed


Optical fiber microarrays have been used to screen saliva from patients with end-stage renal disease (ESRD) to ascertain the efficacy of dialysis. We have successfully identified markers in saliva that correlate with kidney disease. Standard assay chemistries for these markers have been converted to disposable test strips such that patients may one day be able to monitor their clinical status at home. Details of these developments are described. In addition, saliva from asthma and chronic obstructive pulmonary disease (COPD) patients is being screened for useful diagnostic markers. Our goal is to develop a multiplexed assay for these protein and nucleic acid biomarkers for diagnosing the cause and severity of pulmonary exacerbations, enabling more effective treatment to be administered. These results are reported in the second part of this article.

Download full-text


Available from: Eva J Helmerhorst, Sep 17, 2014
37 Reads
  • Source
    • "Considering the relatively high co-existence rate for saliva proteins and their counterpart mRNAs, the salivary transcriptome derived from DNA microarray analyses may serve as a good indicator of the diversity and range of the salivary proteome, and can be used as a reference guideline for human saliva mass spectrometry proteomic profiling [12]. For example optical fiber microarrays have been used to screen saliva from patients with end-stage renal disease (ESRD) to ascertain the efficacy of dialysis, where two salivary analytes (nitrite and uric acid) were successfully identified markers in saliva that correlate with kidney disease that were elevated in predialysis patients and were shown to be reduced following dialysis [13]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Recent advancement in mass spectrometry leads us to a new era of proteomic analysis. Human saliva can be easily collected; however, the complexity of the salivary proteome in the past prevented the use of saliva for proteomic analysis. Here we review the development of proteomic analyses for human saliva and focus on the use of a new mass spectrometric technology known as surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF). SELDI-TOF, a modification of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF), combines the precision of mass spectrometry and the high-through-put nature of protein arrays known as Protein Chips. This technology shows a promising future for salivary proteomic analysis in monitoring treatments and diseases, as well as novel biomarker discovery.
    The Open Dentistry Journal 02/2009; 3(1):74-79. DOI:10.2174/1874210600903010074
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Optical-fiber bundles have been employed as a versatile substrate for the fabrication of high-density microwell arrays. In this minireview, we discuss the application of optical-fiber-bundle arrays for a variety of biological problems. For genomics studies and microbial pathogen detection, individual beads have been functionalized with DNA probes and then loaded into the microwells. In addition, beads differentially responsive to vapors have been employed in an artificial olfaction system. Microwell arrays have also been loaded with living cells to monitor their individual response to biologically active compounds over long periods. Finally, the microwells have been sealed to enclose single enzyme molecules that can be used to measure individual molecule catalytic activity.
    FEBS Journal 12/2007; 274(21):5462-70. DOI:10.1111/j.1742-4658.2007.06078.x · 4.00 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The recovery of steroids, peptides and therapeutic drugs from commercial saliva collection devices was investigated. Saliva, spiked with defined concentrations of the analytes was applied to the Quantisal, three different Salivettes, and the Saliva-Collection-System to investigate effects of volume, exposure time and temperature on the recovery. Additionally, saliva was collected from healthy subjects with the same devices. It was found that glucocorticoids can be measured very well from samples obtained with the synthetic fiber Salivettes and the Quantisal (80-100%). For androgens, the Quantisal and the Saliva-Collection-System reached recoveries >80%. The Quantisal and polyester Salivette achieved best recoveries (>80%) for peptides. The results for the cotton Salivette were extremely poor for melatonin, insulin or IL-8 (<20%). The results from the spike-recovery experiments were confirmed by samples collected from healthy volunteers. For most therapeutic drugs the synthetic fiber Salivettes achieved best recoveries of 100+/-10%. Longer exposure of saliva on the collection devices must be avoided for most of the analytes, due to their limited stability and increased adsorption. In conclusion, no device is suitable for all of the salivary compounds. Strict pre-analytical precautions must be considered (e.g. immediate processing of the sample) to guarantee reliable analytical results.
    Journal of Pharmaceutical and Biomedical Analysis 08/2008; 47(3):478-86. DOI:10.1016/j.jpba.2008.01.033 · 2.98 Impact Factor
Show more