Article

Analgesic and antiinflammatory activity of Cyclamen repandum S. et S.

Department of Pharmacology, via Irnerio 48, Bologna University, 40126 Bologna, Italy.
Phytotherapy Research (Impact Factor: 2.4). 08/2007; 21(7):684-9. DOI: 10.1002/ptr.2145
Source: PubMed

ABSTRACT According to folk medicine some species belonging to the genus Cyclamen were used for their biological activities. Early investigation of the different species of the genus resulted in the isolation of triterpenic saponins. No phytochemical and biological data are available on C. repandum. As part of a series of phytochemical investigations for bioactive compounds from medicinal plants, Cyclamen repandum S. et S. was investigated. The present study sought to find the antiinflammatory and antinociceptive activities of C. repandum tubers in rats and mice. A preliminary screening was conducted with three different extracts in the tests used, particularly the paw edema and the writhing tests. Subsequently some saponins isolated from the ME extract, the more effective one, have been identified. This paper also describes the results of fractionation and bioassay guided chemical studies. Chemical investigation of the active extract afforded the isolation and characterization of six triterpenic saponins. The possible antiinflammatory and analgesic properties were investigated as the saponin content of the fractions allows to speculate on such aspect.

0 Bookmarks
 · 
193 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Context: The genus Cyclamen L. (Primulaceae) is rich in saponins known to have interesting biological activities. Objective: To isolate saxifragifolin B and cyclamin, two triterpene saponins, from Cyclamen libanoticum Hildebr and Cyclamen persicum Mill, and to assess their cytotoxic, clastogenic/aneugenic, and anticlastogenic effects, as well as antioxidant potential. Materials and methods: Saxifragifolin B and cyclamin were tested for their cytotoxicity against SK-BR-3, HT-29, HepG2/3A, NCI-H1299, BXPC-3, 22RV1, and normal DMEM cell lines using WST-1 assay. Their clastogenic/aneugenic activities and anticlastogenic effects against the anticancer drug mitomycin C were assessed by the in vitro micronucleus assay in CHO cells. Their antioxidant capacities were determined using Fe(2+)-chelating and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. Results: Both saponins were described for the first time in Cyclamen libanoticum. They showed strong cytotoxic activities against the tested cancer cell lines. Saxifragifolin B was found to be 56- and 37-times more active than mitomycin C against breast adenocarcinoma (SK-BR-3) and lung carcinoma (NCI-H1299), respectively. Also, saxifragifolin B did not induce micronuclei formation and prevented cells from mitomycin C clastogenic effect. Cyclamin induced a significant increase of micronucleated cells after metabolic activation with S9 mix, and did not possess any anticlastogenic activity. Both molecules exhibited low antioxidant activities as compared to reference compounds. Discussion and conclusions: This study showed the remarkable cytotoxic activity of saxifragifolin B, especially against breast adenocarcinoma and lung carcinoma and its chemoprotective activity against mitomycin C. Thus, saxifragifolin B could be suggested as a potential cytotoxic drug with a preventive effect against possible exposures to genotoxic agents.
    Pharmaceutical Biology 03/2014; · 1.21 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective To examine the effect of water extracts of cyclamen tubers on the expression of main cytochrome P450 (CYP450s) including CYP1A1, CYP1A2 CYP2E1, CYP2B6, CYP2C9 and CYP3A4 that participate in the metabolism of both drugs and carcinogens and cytotoxic activity in human cancer cell lines, namely HepG2 and Caco-2. Methods Cyclamen trochopteranthum tubers were extracted with dH2O and then lyophilized under vacuum. Infrared spectral study was made for extracts by Fourier transform infrared spectroscopy (FT-IR). Cytotoxic activity of cyclamen was determined by crystal violet staining in HepG2 and Caco-2 cells. CYP expression was determined by reverse transcription polymerase chain reaction (RT-PCR). Results Cyclamen water extract had moderate cytotoxic activity. It was found that lethal concentration (LC50) value of the cyclamen extract was 50 and 125 μg/mL in HepG2 and Caco-2 cell lines, respectively. Moreover, it caused induction and suppression of CYP450s mRNA levels in HepG2 and Caco-2 cells. Conclusion Cyclamen may have a potential not only inhibition and/or induction of the metabolism of certain co-administered drugs but also development of toxicity, mutagenesis and malignant transformation due to induction or suppression of the CYP450s dependent drug metabolizing enzymes.
    Chinese Journal of Integrative Medicine 09/2012; · 1.06 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The electron emission yield due to electron impact on polycrystalline Al2O3 is measured with a technique based on the use of a Kelvin probe (KP method) and a pulsed electron beam. The KP method allows the clear discrimination between the external effects of charging and internal ones. The effect of the incident electron fluence on the yield in the region where the yield is higher than one is investigated. An overall drop of the electron emission yield with increasing the electron fluence is observed. This result is clearly associated to the internal effects of positive charging. Indeed, the recombination of the generated secondary electrons with the accumulated holes beneath the irradiated surface leads to the decrease of their mean free path and to the decay of the secondary electron emission yield.
    Applied Surface Science 03/2011; 257(10):4593-4596. · 2.54 Impact Factor