Genetic manipulation of Aspergillus nidulans: Meiotic progeny for genetic analysis and strain construction

Department of Genetics, The University of Melbourne, Parkville, Victoria 3010, Australia.
Nature Protocol (Impact Factor: 8.36). 02/2007; 2(4):811-21. DOI: 10.1038/nprot.2007.112
Source: PubMed

ABSTRACT The multicellular microbial eukaryote Aspergillus nidulans is an excellent model for the study of a wide array of biological processes. Studies in this system contribute significantly to understanding fundamental biological principles and are relevant for biotechnology and industrial applications, as well as human, animal and plant fungal pathogenesis. A. nidulans is easily manipulated using classical and molecular genetics. Here, we describe the storage and handling of A. nidulans and procedures for genetic crossing, progeny analysis and growth testing. These procedures are used for Mendelian analysis of segregation of alleles to show whether a mutant phenotype segregates as a single gene and independent assortment of genes to determine the linkage relationship between genes. Meiotic crossing is used for construction of multiple mutant strains for genetic analysis. Genetic crossing and analysis of progeny can be undertaken in 2-3 weeks and growth testing takes 2-3 days.

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Available from: Michael J Hynes, Jul 28, 2015
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    • "yeasthomologousrecombination(Orr-Weaveretal.,1983)andinte- gratedattheendogenouslocususinghomologousrecombinationinstrains lackingku70(Nayaketal.,2006).Taggedstrainswereconfirmedby PCR.Somestrainswerecreatedthroughgeneticcrosses,aspreviously described(Toddetal.,2007). "
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    • "Transcription factors controlling carbon metabolism 959 described (Todd et al., 2007). Standard methods for DNA manipulations, RNA isolation, nucleic acid blotting and hybridization have been described (Sambrook and Russell, 2001; Hynes et al., 2006). "
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