Article

Effect of basal forebrain neuropeptide Y administration on sleep and spontaneous behavior in freely moving rats.

Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter Sétány 1/C, H-1117 Budapest, Hungary.
Brain Research Bulletin (Impact Factor: 2.94). 06/2007; 72(4-6):293-301. DOI: 10.1016/j.brainresbull.2007.01.006
Source: PubMed

ABSTRACT Neuropeptide Y (NPY) is present both in local neurons as well as in fibers in the basal forebrain (BF), an area that plays an important role in the regulation of cortical activation. In our previous experiments in anaesthetized rats, significant EEG changes were found after NPY injections to BF. EEG delta power increased while power in theta, alpha, and beta range decreased. The aim of the present experiments was to determine whether NPY infusion to BF can modulate sleep and behavior in freely moving rats. In this study, microinjections were made into the BF. Saline was injected to the control side, while either saline or one of two doses of NPY (0.5 microl, 300-500 pmol) to the treated side. EEG as well as behavioral changes were recorded. Behavioral elements after the NPY injections changed in a characteristic fashion in time and three consecutive phases were defined. In phase I (half hour 2), activated behavioral items (moving, rearing, grooming) appeared frequently. In phase II (half hours 3 and 4) activity decreased, while motionless state increased. Reappearance of activity was seen in phase III (half hours 5 and 6). NPY injections caused sleep-wake changes. The three phases described for behavioral changes were also reflected in the sleep data. During phase I, lower NPY dose increased wakefulness and decreased deep sleep. Reduced behavioral activity seen in phase II was partially reflected in the sleep. In this phase, wakefulness tended to increase in the third half hour, while decreased in the 4th half hour. Deep sleep and total slow wave sleep non-significantly decreased in the third and increased in the 4th half hour. In most cases, wakefulness was elevated again during Phase III, while sleep decreased. Length of single sleep-wake epochs did not change after NPY injections. Our results suggest a role for NPY in the integration of sleep and behavioral stages via the BF.

1 Bookmark
 · 
94 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Sleep is important for maintenance of normal physiology in animals. In mammals, neuropeptide Y (NPY), a homolog of Drosophila neuropeptide F (NPF), is involved in sleep regulation, with different effects in human and rat. However, the function of NPF on sleep in Drosophila melanogaster has not yet been described. In this study, we investigated the effects of NPF and its receptor-neuropeptide F receptor (NPFR1) on Drosophila sleep. Male flies over-expressing NPF or NPFR1 exhibited increased sleep during the nighttime. Further analysis demonstrated that sleep episode duration during nighttime was greatly increased and sleep latency was significantly reduced, indicating that NPF and NPFR1 promote sleep quality, and their action on sleep is not because of an impact of the NPF signal system on development. Moreover, the homeostatic regulation of flies after sleep deprivation was disrupted by altered NPF signaling, since sleep deprivation decreased transcription of NPF in control flies, and there were less sleep loss during sleep deprivation and less sleep gain after sleep deprivation in flies overexpressing NPF and NPFR1 than in control flies, suggesting that NPF system auto-regulation plays an important role in sleep homeostasis. However, these effects did not occur in females, suggesting a sex-dependent regulatory function in sleep for NPF and NPFR1. NPF in D1 brain neurons showed male-specific expression, providing the cellular locus for male-specific regulation of sleep by NPF and NPFR1. This study brings a new understanding into sleep studies of a sexually dimorphic regulatory mode in female and male flies.
    PLoS ONE 01/2013; 8(9):e74237. · 3.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract The monitoring ,of neurochemicals represents a ,challenge ,for current analytical techniques due to a variety of factors such as compositional complexity, limited sample- amounts,and ,endogenous ,inferences. Advances ,in MS ,provide ,great opportunities for sensitive in vivo monitoring of neurochemicals, offering benefits including simple sample preparation, broad capability for analysis of structurally diverse compounds and rich structural information of analytes. MS has alsobecome,an indispensabletool for neuropeptide analysis, offering tremendous potential in the discovery of novel signaling peptides and biomarkers. This review covers recent advances in MS-based neurochemical,measurements including a comparison with related techniques, neuropeptide discovery and chromatographic separation. Issues relating to in vivo sample collection and sample preparation are discussed. Wehighlight,perspectives for the use of MS for neurochemical ,analysis in non-human primates. Key words: neurochemical analysis, mass spectrometry, HILIC, neurotransmitter, neuropeptide, non-human primate, EBF, biomarker ,3
  • Source
    The Mouse Nervous System, First Edition edited by Charles Watson, George Paxinos, Luis Puelles, 01/2012: chapter The Basal Forebrain Cholinergic Projection System in Mice Neuron Types in the Basal Forebrain - Chapter 28: pages 684–718; Academic Press Elsevier., ISBN: 978-0-12-369497-3

Full-text (3 Sources)

Download
3 Downloads
Available from
Sep 16, 2014

Laszlo Zaborszky