Oude Engberink, R.D., et al.: Comparison of SPIO and USPIO for in vitro labeling of human monocytes: MR detection and cell function. Radiology 243, 467-474

Department of Molecular Cell Biology and Immunology, VU University Amsterdam, Amsterdamo, North Holland, Netherlands
Radiology (Impact Factor: 6.21). 05/2007; 243(2):467-74. DOI: 10.1148/radiol.2432060120
Source: PubMed

ABSTRACT To label human monocytes with superparamagnetic iron oxide (SPIO) and compare labeling efficiency with that of ultrasmall SPIO (USPIO) and evaluate the effect of iron incorporation on cell viability, migratory capacity, and proinflammatory cytokine production.
The study was approved by the institutional ethics committee; informed consent was obtained from donors. Freshly isolated human monocytes were labeled with iron particles of two sizes, USPIOs of 30 nm and SPIOs of 150 nm, for 1.5 hours in culture medium containing 0.1, 0.5, 1.0, and 3.7 mg of iron per milliliter. Labeling efficiency was determined with relaxation time magnetic resonance (MR) imaging (4.7 T) and Prussian blue staining for presence of intracellular iron. Cell viability was monitored; migratory capacity of monocytes after labeling was evaluated by using an in vitro assay with monolayers of brain endothelial cells. Levels of proinflammatory cytokines, interleukin (IL) 1 and IL-6, were measured with enzyme-linked immunosorbent assay 24 hours after labeling. Data were analyzed with Student t test or two-way analysis of variance followed by a multiple-comparison procedure.
R2 relaxation rates increased for cell samples incubated with SPIOs, whereas rates were not affected for samples incubated with highest concentration of USPIOs. Labeling monocytes with SPIOs (1.0 mg Fe/mL) resulted in an R2 of 13.1 sec(-1) +/- 0.8 (standard error of the mean) (7 sec(-1) +/- 0.2 for vehicle-treated cells, P < .05) and had no effect on cell viability. On the basis of T2 relaxation times, the in vitro MR detection limit of 58 labeled monocytes per 0.05 microL was calculated. Migration of labeled monocytes was not different from that of vehicle-treated cells. Intracellular iron had no effect on production of IL-1 and IL-6 24 hours after labeling.
In vitro labeling of human monocytes is effective by using SPIOs, not USPIOs. Incubation with SPIOs (1.0 mg Fe/mL) results in efficient labeling detectable on MR images and does not affect cellular viability and activation markers such as cell migration and cytokine production.

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    • "Iron oxide MNP with total size of 100 nm were preferred in this study as MNP in this range have been shown to be more effective for in vitro monocyte / macrophage labeling compared to smaller nanoparti - cles size ( Oude Engberink et al . 2007 ) . Zeta potential analysis has revealed that SPIO , SPIO – PEG , and SPIO – PEG – COOH have a surface charge of - 1 . 8 , - 3 . 3 , and - 5 . 8 mV , respectively . Ferrozine - based spectro - photometry ( Fig . 1 ) revealed higher uptake of iron by M2 macrophages compared to M1 subsets for the different SPIO nanoparticles ( i . e . , a"
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    • "Although there are claims of detection at 10 ¡12 M (Gao and Hillebrenner 2008), present-day technologies involve a trade-oV between the SPIO imaging parameters including Weld strength, gradient characteristics, acquisition time (Kircher et al. 2003; Heyn et al. 2006) against the number of cells to be detected. Also there are diVerences in detection sensitivities between SPIOs and the ultrasmall SPIOs (USPIOs) (Engberink et al. 2007). Nano-and microparticles in the form of iron oxide particles are available commercially in streptavidin-conjugated form and can be used to target tumour cells (Artemov et al. 2003). "
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