Article

Human hepatocyte morphology and functions in a multibore fiber bioreactor.

Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
Macromolecular Bioscience (impact factor: 3.89). 06/2007; 7(5):671-80. DOI:10.1002/mabi.200600281 pp.671-80
Source: PubMed

ABSTRACT The viability and liver specific functions of human hepatocytes in a multibore fiber bioreactor are reported. Human hepatocytes were cultured in the intraluminal compartment of the bioreactor. Human hepatocytes on the membranes maintained their round shape and showed focal adhesions as sites of interaction with the membrane surface. Cells in the bioreactor expressed liver specific functions, including synthetic and detoxification activity up to 14 d of culture. The results demonstrate that human hepatocytes cultured in the multibore fiber bioreactor are able to sustain the same in vivo liver functions in vitro.

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Keywords

bioreactor
 
detoxification activity
 
focal adhesions
 
human hepatocytes
 
human hepatocytes cultured
 
intraluminal compartment
 
liver specific functions
 
membranes
 
multibore fiber bioreactor
 
synthetic
 
vitro
 
vivo liver functions