Proportion of peripheral blood and decidual CD4 CD25 regulatory T cells in pre-eclampsia

Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan.
Clinical & Experimental Immunology (Impact Factor: 3.28). 07/2007; 149(1):139-45. DOI: 10.1111/j.1365-2249.2007.03397.x
Source: PubMed

ABSTRACT CD4(+) CD25(bright) regulatory T (T(reg)) cells have been identified as a principle regulator of tolerance during pregnancy. In the setting of pre-eclampsia, however, little is known about the dynamics of these cells. In the current study, we determined CD4(+) CD25(bright) T(reg) cells in the peripheral blood using flow cytometry and forkhead box P3 (FoxP3(+)) cells at the placental bed using immunohistochemical staining. Peripheral blood mononuclear cells (PBMC) of 38 pre-eclamptic cases (17 cases Japanese, 21 cases Polish), 40 normal late pregnancy subjects (20 subjects Japanese, 20 subjects Polish), and 21 non-pregnant healthy controls (10 subjects Japanese, 11 subjects Polish) were included. We found the percentage of CD25(bright) cells within the CD4(+) T cell population in PBMC was reduced significantly in both Japanese and Polish pre-eclamptic cases than in normal pregnancy subjects (P < 0.001) and non-pregnant healthy controls (P < 0.001). Also, the percentage of FoxP3(+) cells within CD3(+) T cells in the placental bed biopsy samples of pre-eclamptic cases were decreased compared to those in normal pregnancy subjects. These findings suggest that a decreased number of T(reg) cells was present in pre-eclampsia, and these changes might break the maternal tolerance to the fetus.

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Available from: Arihiro Shiozaki, Feb 04, 2015
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    • "In humans, too, Tregs are increased in the decidua during pregnancy (Sasaki et al., 2004). In women with complicated pregnancies, decreased numbers of Tregs were found in the decidua (Yang et al., 2008) and placenta (Sasaki et al., 2007) suggesting that these Tregs might play a pivotal role in uncomplicated pregnancies. Other studies in mice have shown that during copulation , long before implantation, maternal tolerance toward 0165-0378/© 2015 Elsevier Ireland Ltd. "
    Journal of Reproductive Immunology 03/2014; s 101–102:20. DOI:10.1016/j.jri.2013.12.079 · 2.37 Impact Factor
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    • "and adaptive immune system that may have an influence on the onset of this disorder. It was suggested that activation of cell-mediated immunity might play a key role in the aetiology of pre-eclampsia (Darmochwal-Kolarz et al., 2007; Kalkunte et al., 2009; Saito and Sakai, 2003; Santner- Nanan et al., 2009; Sasaki et al., 2007). Dendritic cells (DCs) are antigen-presenting cells with the unique ability to induce primary immune responses. "
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    ABSTRACT: The aim of our study was to estimate the expression of B7-H1 and B7-H4 molecules on myeloid and plasmacytoid dendritic cells (DCs) in the peripheral blood of patients with pre-eclampsia, normal pregnant women and healthy non-pregnant women. Thirty-three patients with pre-eclampsia, 26 normal pregnant women, and 12 healthy non-pregnant women were included in the study. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens and B7-H1 and B7-H4 molecules and estimated using flow cytometry. The expression of B7-H1 and B7-H4 molecules was significantly higher on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy than in the luteal phase of the ovarian cycle (CD1c(+)B7-H1(+): 19.19±10.55% vs. 11.99±6.79%; p<0.05; CD1c(+)B7-H4(+): 12.01±9.15% vs. 3.98±1.97%, p<0.001; CD303(+)B7-H1(+): 4.15±2.38% vs. 1.70±0.87%, p<0.05; CD303(+)B7-H4(+): 5.44±2.93% vs. 2.33±1.54%, p<0.01). Moreover, the expression of the B7-H1 molecule on CD1c(+) DCs in the second trimester of normal pregnancy was significantly higher than in the first trimester, but in the third trimester they decreased compared with the second trimester (II vs. I trimester: 32.23±11.30% vs. 19.19±10.55%, p<0.01; III vs. II trimester: 32.23±11.30% vs. 22.39±8.19%, p<0.01). The expression of B7-H1 molecule on CD1c(+) myeloid and CD303(+) plasmacytoid DCs was significantly lower in pre-eclampsia than in healthy third-trimester pregnant women (CD1c(+)B7-H1(+): 13.78±6.26% vs. 22.39±8.19%, p<0.05; CD303(+)B7-H1(+): 3.66±2.46% vs. 8.65±3.15%, p<0.01). Higher expressions of B7-H1 and B7-H4 molecules on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy suggest the role they play in the immunomodulation during early pregnancy.
    Journal of Reproductive Immunology 06/2013; 99(1-2). DOI:10.1016/j.jri.2013.04.004 · 2.37 Impact Factor
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    • "The importance of Treg cells in pregnancy is further supported by studies of individuals who had complications of pregnancy. In preeclampsia, CD4 + CD25 high Treg cells are significantly reduced in both peripheral blood and decidual tissue compared to normal pregnant women (Sasaki et al., 2007). In spontaneous abortions, the levels of CD4 + CD25 + Treg cells are significantly lower in patients who had a spontaneous abortion compared to samples from induced abortions (Sasaki et al., 2004). "
    Recent Advances in Research on the Human Placenta, 03/2012; , ISBN: 978-953-51-0194-9
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