Ultrastructural findings in murine seminiferous tubules as a consequence of subchronic vanadium pentoxide inhalation.
ABSTRACT Vanadium (V) is a transition metal emitted to the atmosphere during the combustion of fossil fuels. Its current status as an atmospheric pollutant increases the need for information about the effects that this element might have on the reproductive health of exposed populations. The present study investigated changes in testicular ultrastructure following inhalation exposure of male mice to V (as vanadium pentoxide). Tissue V level was constant during the 12-week time period. We observed necrosis of spermatogonium, spermatocytes and Sertoli cells, as well as pseudo-nuclear inclusion and disruption of cellular junctions. Our findings stressed the importance of the hemato-testicular barrier in supporting the function of Sertoli cells and suggest as a possible target of V, tight junction proteins. Further analysis is needed in order to identify the role that reactive oxidative species (ROS) might have on these cellular junctions, and if a specific protein is the target of its toxic effects. The relevance of this report concerns the impact that metal air pollution could have on male fertility in dense cities with vehicular traffic problems.
- SourceAvailable from: Juan José Rodriguez[Show abstract] [Hide abstract]
ABSTRACT: Vanadium and vanadium salts cause genotoxicity and elicit variable biological effects depending on several factors. In the present study, we analyzed and compared the DNA damage and repair processes induced by vanadium in three oxidation states. We used human blood leukocytes in vitro and in a single cell gel electrophoresis assay at two pH values. We observed that vanadium(III) trioxide and vanadium(V) pentoxide produced DNA single-strand breaks at all of the concentrations (1, 2, 4, or 8 μg/ml) and treatment times (2, 4, or 6 h) tested. Vanadium(IV) tetraoxide treatment significantly increased DNA damage at all concentrations for 4 or 6 h of treatment but not for 2 h of treatment. The DNA repair kinetics indicated that most of the cells exposed to vanadium III and V for 4 h recovered within the repair incubation time of 90 min; however, those exposed to vanadium(IV) repaired their DNA within 120 min. The data at pH 9 indicated that vanadium(IV) tetraoxide induced DNA double-strand breaks. Our results show that the genotoxic effect of vanadium can be produced by any of its three oxidation states. However, vanadium(IV) induces double-strand breaks, and it is known that these lesions are linked with forming structural chromosomal aberrations.Toxicology in Vitro 07/2011; 25(8):1996-2002. DOI:10.1016/j.tiv.2011.07.009 · 3.21 Impact Factor
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ABSTRACT: Atmospheric pollution has been a nightmare in human technological development, which is being reflected worldwide. The worst scenarios are located in developing countries such as those located in Latin America, Asia and Africa. An interesting phenomena has been reported by Campell and Campell (2007) that they defines as "flattening of the cities" which is related with the spread of human settlements in periurban regions far from the city and health care facilities; also these settlements promote erosion, which facilitates particulate air pollution. This changes increase the need for transportation and the increase in traffic jams, as well as the accretion of atmospheric pollutants. The Vehicular Traffic Factor The health impact that inhaled particulate matter has on human health and its association with myocardial infarctions, thromboembolic diseases and other related health problems has increase. Peters et al. (2004) identified the association of traffic-related air pollution and an increase in myocardial infarction events. Some factors increased de risk such as strenuous exercise, and there were no differences if the subject was riding a bicycle, or being transported in public transportation, made no difference. The report mentioned that women and subjects over 60 years of age or older, and patients with diabetes were at higher risk for the onset of an infarction when compared with men, non diabetic or subjects younger 60 years old, after the exposure to traffic. Stress, air pollution, and noise, as well as, individual The exclusive license for this PDF is limited to personal website use only. No part of this digital document may be reproduced, stored in a retrieval system or transmitted commercially in any form or by any means. The publisher has taken reasonable care in the preparation of this digital document, but makes no expressed or implied warranty of any kind and assumes no responsibility for any errors or omissions. No liability is assumed for incidental or consequential damages in connection with or arising out of information contained herein. This digital document is sold with the clear understanding that the publisher is not engaged in rendering legal, medical or any other professional services.Traffic Related Air Pollution and Internal Combustion Engines, 1 edited by Sergey Demidov and Jacques Bonnet, 01/2009: chapter 1: pages 1-28; Nova Publishers., ISBN: 978-1-60741-145-1
Conference Paper: A new approach for the global analysis of fuzzy dynamical systems[Show abstract] [Hide abstract]
ABSTRACT: A novel approach for analyzing the global behavior of a fuzzy dynamical system is presented. It applies the concept and method of the cell-to-cell mapping to obtain the evolving trend of the states of the fuzzy dynamical system. The behavior of system is characterized by equilibria, periodic motions, and their domain of attractions. The min-max operation accumulates the fuzziness of a fuzzy system in every iteration and makes state evolution obscure. The proposed method transforms a given fuzzy mapping to a Z -to- Z mapping and does not accumulate fuzziness. Both the real and fuzzy initial state response analyses are discussed. An inverted pendulum controlled by a fuzzy controller is analyzed by the method to illustrate its validityDecision and Control, 1988., Proceedings of the 27th IEEE Conference on; 01/1989