Integration of genomics, proteomics, and imaging for cardiac stem cell therapy

Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, CA, USA.
European journal of nuclear medicine and molecular imaging (Impact Factor: 5.38). 07/2007; 34 Suppl 1(S1):S20-6. DOI: 10.1007/s00259-007-0437-y
Source: PubMed


Cardiac stem cell therapy is beginning to mature as a valid treatment for heart disease. As more clinical trials utilizing stem cells emerge, it is imperative to establish the mechanisms by which stem cells confer benefit in cardiac diseases. In this paper, we review three methods - molecular cellular imaging, gene expression profiling, and proteomic analysis - that can be integrated to provide further insights into the role of this emerging therapy.

2 Reads
  • Source
    • "Reporter gene technology has a number of advantages over direct labeling of cells with imaging agents (Chun et al., 2007). Firstly, reporter gene imaging requires cellular viability. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The convergence of molecular and genetic disciplines with non-invasive imaging technologies has provided an oppor-tunity for earlier detection of disease processes which begin with molecular and cellular abnormalities. This emerging field, known as molecular imaging, is a relatively new dis-cipline that has been rapidly developed over the past decade. It endeavors to construct a visual representation, character-ization, and quantification of biological processes at the mo-lecular and cellular level within living organisms. One of the goals of molecular imaging is to translate our expanding knowledge of molecular biology and genomic sciences into good patient care. The practice of molecular imaging is still largely experimental, and only limited clinical success has been achieved. However, it is anticipated that molecular imaging will move increasingly out of the research laboratory and into the clinic over the next decade. Non-invasive in vivo molecular imaging makes use of nuclear, magnetic reso-nance, and in vivo optical imaging systems. Recently, an inter-est in Positron Emission Tomography (PET) has been re-vived, and along with optical imaging systems PET is assum-ing new, important roles in molecular genetic imaging studies. Current PET molecular imaging strategies mostly rely on the detection of probe accumulation directly related to the physi-ology or the level of reporter gene expression. PET imaging of both endogenous and exogenous gene expression can be achieved in animals using reporter constructs and radio-labeled probes. As increasing numbers of genetic markers become available for imaging targets, it is anticipated that a better understanding of genomics will contribute to the ad-vancement of the molecular genetic imaging field. In this re-port, the principles of non-invasive molecular genetic imag-ing, its applications and future directions are discussed.
    07/2007; 5:46-55.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Several equations of mixing rule on permittivity have been proposed, but none of these equations is not perfect, because of too many different configurations exist in actual cases. Serial model and parallel model are two extremes of mixing manner. For the random mixture of fine particles, which does not have a remarkable aspect ratio, customarily the logarithmic mixing rule has been applied. But, the logarithmic mixing rule does not estimate the correct apparent permittivity in low or high mixing rate. The author proposed new equation for the mixing rule that gives better agreement with measured value for whole range of mixing rate compared to the logarithmic rule. In this paper, a desirable refinement on the equation in previous paper is made to adapt to the structural image of actual compound and then the equation has been expanded to the complex permittivity to apply the equation on the dissipative materials cases.
    Applications of Ferroelectrics, 2002. ISAF 2002. Proceedings of the 13th IEEE International Symposium on; 01/2002
  • [Show abstract] [Hide abstract]
    ABSTRACT: Stem cell therapy holds enormous potential for treating a wide range of genetic and sporadic degenerative disorders. However, one of the major hurdles facing stem cell therapy is the ability to assess cell fate or outcome prior to transplantation. Recent studies have shown that time-lapse microscopy may be a useful tool to assess cell fate via observation of dynamic behavior at the single-cell and population levels. The ideal embodiment of time-lapse microscopy would be a high-throughput, noninvasive device that can identify stem cells that form nontumorigenic differentiated progeny capable of integration into mature tissues. Such technologies are on the horizon and hold promise for clinical and therapeutic applications. KeywordsImaging-Time-lapse microscopy-Stem cells
    12/2010: pages 181-191;
Show more