Integration of genomics, proteomics, and imaging for cardiac stem cell therapy.
ABSTRACT Cardiac stem cell therapy is beginning to mature as a valid treatment for heart disease. As more clinical trials utilizing stem cells emerge, it is imperative to establish the mechanisms by which stem cells confer benefit in cardiac diseases. In this paper, we review three methods - molecular cellular imaging, gene expression profiling, and proteomic analysis - that can be integrated to provide further insights into the role of this emerging therapy.
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ABSTRACT: The convergence of molecular and genetic disciplines with non-invasive imaging technologies has provided an oppor-tunity for earlier detection of disease processes which begin with molecular and cellular abnormalities. This emerging field, known as molecular imaging, is a relatively new dis-cipline that has been rapidly developed over the past decade. It endeavors to construct a visual representation, character-ization, and quantification of biological processes at the mo-lecular and cellular level within living organisms. One of the goals of molecular imaging is to translate our expanding knowledge of molecular biology and genomic sciences into good patient care. The practice of molecular imaging is still largely experimental, and only limited clinical success has been achieved. However, it is anticipated that molecular imaging will move increasingly out of the research laboratory and into the clinic over the next decade. Non-invasive in vivo molecular imaging makes use of nuclear, magnetic reso-nance, and in vivo optical imaging systems. Recently, an inter-est in Positron Emission Tomography (PET) has been re-vived, and along with optical imaging systems PET is assum-ing new, important roles in molecular genetic imaging studies. Current PET molecular imaging strategies mostly rely on the detection of probe accumulation directly related to the physi-ology or the level of reporter gene expression. PET imaging of both endogenous and exogenous gene expression can be achieved in animals using reporter constructs and radio-labeled probes. As increasing numbers of genetic markers become available for imaging targets, it is anticipated that a better understanding of genomics will contribute to the ad-vancement of the molecular genetic imaging field. In this re-port, the principles of non-invasive molecular genetic imag-ing, its applications and future directions are discussed.07/2007; 5:46-55.
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ABSTRACT: Stem cell therapy holds enormous potential for treating a wide range of genetic and sporadic degenerative disorders. However, one of the major hurdles facing stem cell therapy is the ability to assess cell fate or outcome prior to transplantation. Recent studies have shown that time-lapse microscopy may be a useful tool to assess cell fate via observation of dynamic behavior at the single-cell and population levels. The ideal embodiment of time-lapse microscopy would be a high-throughput, noninvasive device that can identify stem cells that form nontumorigenic differentiated progeny capable of integration into mature tissues. Such technologies are on the horizon and hold promise for clinical and therapeutic applications. KeywordsImaging-Time-lapse microscopy-Stem cells12/2010: pages 181-191;
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ABSTRACT: Heart disease continues to be a leading cause of death in Western world. While strides have been made in prevention and management of coronary artery disease, lost myocardium after an ischemic event remains at the core of the morbidity and the mortality. Poor regenerative capacity of the myocardium has led to the study of cell based therapies to restore anatomical, functional and viable myocardium. To that end, stem cells are undifferentiated cells that are self-renewing, clonogenic and pluripotent and therefore ideal for the restorative job. However, in order to refine the technique of cell-based therapy, in vivo molecular assessment is imperative to monitor cell survival and their impact on myocardial restoration. Direct imaging of the behavior of cells after implantation into living subjects can offer great insights into their mechanisms of action, as well as their therapeutic efficacy. This article explores current knowledge of various imaging modalities that have been used to assess in vivo cellular and molecular events after administration of stem cells in injured myocardium. The goal of the article is to provide comprehensive overview of the literature, highlighting various imaging modalities and suggesting some of the key concepts at the horizon in cardiac stem cell imaging.The Canadian journal of cardiology 11/2014; 30(11). DOI:10.1016/j.cjca.2014.08.028 · 3.94 Impact Factor