Article

Comparison of GC-MS and LC-MS methods for the analysis of antioxidant phenolic acids in herbs.

Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland.
Analytical and Bioanalytical Chemistry (Impact Factor: 3.66). 07/2007; 388(4):881-7. DOI: 10.1007/s00216-007-1298-8
Source: PubMed

ABSTRACT Two methods were developed for the quantitative analysis of phenolic acids in herb extracts. The methods were based on liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) and gas chromatography-mass spectrometry (GC-MS). The methods were compared in terms of their linearity, repeatability, selectivity, sensitivity and the speed of the analysis. The sensitivity was good for both methods, with limits of detection of <80 ng/ml for most of the compounds. The relative standard deviations (RSD) of the peak areas were on average 7.2% for the LC-TOFMS method and 1.4% for the GC-MS method. Both methods were found to be suitable for the determination of the target analytes, although GC-MS was better suited to the quantitative determination of compounds present at low concentrations.

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