Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella. Int J Syst Evol Microbiol

University of Dundee, Dundee, Scotland, United Kingdom
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 06/2007; 57(Pt 5):1055-9. DOI: 10.1099/ijs.0.64873-0
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Three strains, Br3437(T), Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437(T)) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437(T) (=LMG 23741(T)=BCRC 17575(T)), was isolated from nodules of M. scabrella.

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Available from: Euan Kevin James, Sep 30, 2015
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    • "Burkholderia species were isolated from highly diverse habitats (Coenye and Vandamme 2003; Caballero-Mellado et al. 2004; Reis et al. 2004; Chen et al. 2007). Burkholderia is a genus with a steady growth of species numbers. "
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    ABSTRACT: A metal-resistant and phosphate-solubilising bacterium, designated as strain D414(T), was isolated from heavy metal (Pb, Cd, Cu and Zn)-polluted paddy soils at the surrounding area of Dabao Mountain Mine in Southeast China. The minimum inhibitory concentrations of heavy metals for strain D414(T) were 2000 mg L(-1) (Cd), 800 mg L(-1) (Pb), 150 mg L(-1) (Cu) and 2500 mg L(-1) (Zn). The strain possessed plant growth-promoting properties, such as 1-aminocyclopropane-1-carboxylate assimilation, indole production and phosphate solubilisation. Analysis of 16S rRNA gene sequence indicated that the isolate is a member of the genus Burkholderia where strain D414(T) formed a distinct phyletic line with validly described Burkholderia species. Strain D414(T) is closely related to Burkholderia tropica DSM 15359(T), B. bannensis NBRC E25(T) and B. unamae DSM 17197(T), with 98.5, 98.3 and 98.3 % sequence similarities, respectively. Furthermore, less than 34 % DNA-DNA relatedness was detected between strain D414(T) and the type strains of the phylogenetically closest species of Burkholderia. The dominant fatty acids of strain D414(T) were C14:0, C16:0, C17:0 cyclo and C18:1 ω7c. The DNA G+C content was 62.3 ± 0.5 mol%. On the basis of genotypic, phenotypic and phylogenetic data, strain D414(T) represents a novel species, for which the name Burkholderia metalliresistens sp. nov. is proposed, with D414(T) (=CICC 10561(T) = DSM 26823(T)) as the type strain.
    Antonie van Leeuwenhoek 04/2015; 107(6). DOI:10.1007/s10482-015-0453-z · 1.81 Impact Factor
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    • "Of these four, only B. tuberum nodulates legumes, but so far a model plant for the detailed study of this nitrogen-fixing, betaproteobacterial species has not been developed. Other legume-nodulating Burkholderia species include B. phymatum STM815 T (Vandamme et al. 2002), B. nodosa Br3437 T , Br3641, Br2470 (Chen et al. 2007), and a number of B. mimosarum strains (Chen et al. 2006). The latter three species nodulate Mimosa spp. "
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    ABSTRACT: Background and aims Burkholderia tuberum STM678T was isolated from a South African legume, but did not renodulate this plant. Until a reliable host is found, studies on this and other interesting beta-rhizobia cannot advance. We investigated B. tuberum STM678T’s ability to induce Fix+ nodules on a small-seeded, easy-to-propagate legume (Macroptilium atropurpureum). Previous studies demonstrated that B. tuberum elicited either Fix- or Fix+ nodules on siratro, but the reasons for this difference were unexplored. Methods Experiments to promote effective siratro nodule formation under different environmental conditions were performed. B. tuberum STM678T’s ability to withstand high temperatures and desiccation was checked as well as its potential for promoting plant growth via mechanisms in addition to nitrogen fixation, e.g., phosphate solubilization and siderophore production. Potential genes for these activities were found in the sequenced genomes. Results Higher temperatures and reduced watering resulted in reliable, effective nodulation on siratro. Burkholderia spp. solubilize phosphate and produce siderophores. Genes encoding proteins potentially involved in these growth-promoting activities were detected and are described. Conclusions Siratro is an excellent model plant for B. tuberum STM678T. We identified genes that might be involved in the ability of diazotrophic Burkholderia species to survive harsh conditions, solubilize phosphate, and produce siderophores.
    Plant and Soil 08/2013; 369(1-2). DOI:10.1007/s11104-013-1590-7 · 2.95 Impact Factor
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    • ") B. mimosarum Mimosa spp. Chen et al. (2006) B. nodosa Mimosa bimucronata Chen et al. (2007) Mimosa scabrella B. phymatum Tropical mimosoid woody legumes Vandamme et al. (2000) B. sabiae Mimosa caesalpiniifolia Chen et al. (2008) B. tuberum Tropical mimosoid woody legumes Vandamme et al. (2000), Moulin et al. (2001) (continued) promiscuous host plant since the capability of nodulate P. vulgaris effectively is present in a genetically heterogeneous group of bacteria originating from all over the world (Laguerre et al. 1993). Historically, P. vulgaris was the first legume in which Rhizobium symbiosis was identified (Taylor et al. 1983), and the original microsymbiont of P. vulgaris is Rhizobium etli (Segovia et al. 1993). "
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    ABSTRACT: In the Himalayan climatic condition where temperature are crucial determinant for microbial growth as well as the growth of plant, productivity of important crops can be increased by the use of cold-tolerant rhizospheric microflora. Crop enhancement of legumes found in the western Himalaya has been reviewed in this chapter with special emphasis on Rhizobium spp. These are characterized to retain their plant growth promotory activity in suboptimal temperature conditions. Psychrotolerant rhizobia which can grow over a wide temperature range from 4 to 42 °C and usually grow optimally at temperature above 20 °C are extremely important, since they have survive and retain their functionality in low-temperature area such as Himalaya mountain ranges in India. Potential of Rhizobia in productivity enhancement of Macrotyloma uniflorum L. and Phaseolus vulgaris L., the two important legumes cultivated in high altitude regions of the western Himalaya have been highlighted. Besides nitrogen fixation, the role of other PGP attributes in indigenous strains with reference to phosphate solubilization, phytohormones promotion, and siderophore production has also been analyzed in raising crop’s yield. The taxonomy of rhizobial isolates has been included which would be helpful to explore current status of rhizobia. Knowledge of the biodiversity of Rhizobium from local crops is discussed for the design of successful inoculations leading to increased yield in legumes.
    Bacteria in Agrobiology: Crop Productivity, Edited by Dinesh K Maheshwari, Abhinav Aeron, Meenu Saraf, 05/2013: chapter 6: pages 127-165; Springer Berlin Heidelberg., ISBN: 978-3-642-37240-7
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