Article

Maturation of the mammalian secretome

Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Genome biology (Impact Factor: 10.47). 02/2007; 8(4):211. DOI: 10.1186/gb-2007-8-4-211
Source: PubMed

ABSTRACT A recent use of quantitative proteomics to determine the constituents of the endoplasmic reticulum and Golgi complex is discussed in the light of other available methodologies for cataloging the proteins associated with the mammalian secretory pathway.

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Available from: Jeremy C Simpson, Sep 02, 2015
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    • "The term secretome is often used to refer to the complete set of secreted proteins in an organism (2,11,12). However, the term has also been used to include the set of proteins involved in the secretory pathway (13,14). In the work described here, the secretome only includes the secreted proteins in an organism. "
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    • "In eukaryotes, the term secretome has been used to describe different subsets of the proteome, including (1) all the proteins processed through the secretory pathway (Klee, 2008), (2) the proteins processed through the secretory pathway that lack transmembrane domains and/or a glycosylphosphatidylinositol anchor signal (Grimmond et al., 2003; Lee et al., 2003), or (3) the subset of proteins identified in the extracellular proteome (Zwickl et al., 2005; Chevallet et al., 2007; Paper et al., 2007). From a proteomic perspective, the mammalian secretome was defined as the quantitative map for the distribution of all proteins and lipids in the classical secretory pathway (Simpson et al., 2007). These studies reveal that the term 'secretome' has been used (or misused) in a variety of ways. "
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