Phenotypic and phylogenic groups to evaluate the diversity of Citrobacter isolates from activated biomass of effluent treatment plants.
ABSTRACT The diversity of Citrobacter isolated from effluent treatment plants (ETPs) was studied using three different parameters. Thirty Citrobacter strains were isolated from different ETPs treating wastewaters generated at various industries. All the isolates were characterized based on biochemical tests, antibiotic assay/functional analysis, and phylogenetic analysis. Results demonstrated that the pattern of grouping varied based on the selected criteria for analysis. Species that clustered together by biochemical analysis were found to vary by functional and 16S rDNA analysis and vice versa. This suggests that multiple methods approach needs to be carried out to understand the microbial diversity. Bacteria in effluent treatment plants are exposed to diverse categories of pollutants. Salicylate is a key intermediate formed during biodegradation of several aromatic compounds, a scenario expected in ETPs. Hence, the Citrobacter isolates were screened for their capability to utilize salicylate. In future studies, these isolates can be incorporated in a bioremediation program.
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ABSTRACT: An ad hoc committee for the re-evaluation of the species definition in bacteriology met in Gent, Belgium, in February 2002. The committee made various recommendations regarding the species definition in the light of developments in methodologies available to systematists.International journal of systematic and evolutionary microbiology 06/2002; 52(Pt 3):1043-7. · 2.11 Impact Factor
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ABSTRACT: All living organisms fall into discrete clusters of closely related individuals on the basis of gene sequence similarity. Evolutionary genetic theory predicts that in the bacterial world, each sequence similarity cluster should correspond to an ecologically distinct population. Indeed, surveys of sequence diversity in protein-coding genes show that sequence clusters correspond to ecological populations. Future population surveys of protein-coding gene sequences can be expected to disclose many previously unknown ecological populations of bacteria. Sequence similarity clustering in protein-coding genes is recommended as a primary criterion for demarcating taxa.International journal of systematic bacteriology 11/1997; 47(4):1145-56. · 2.27 Impact Factor
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ABSTRACT: This paper describes a method that facilitates the extraction of PCR-compatible DNA from different activated sludge samples. The approach involves a novel preprocessing step in DNA extraction, which removes potential PCR inhibitors. The sludge was washed with different ratios of acetone and petroleum ether after pretreatment with 0.01% Tween-20 at 50 degrees C. It was observed that an initial washing step with 50 mM Tris-HCl, pH 9.0, before the detergent-solvent step, improved the quality of the extracted DNA. The extraction protocol resulted in amplifiable amounts of DNA when 10 mg of a sludge sample was used, even in the presence of phenol as a sludge contaminant. The usefulness of the extracted template was demonstrated by carrying out different PCR reactions. The random amplified polymorphic DNA (RAPD) patterns demonstrated the diversity of sludge samples.Journal of Microbiological Methods 04/2003; 52(3):315-23. · 2.16 Impact Factor