Article

Phenotypic and phylogenic groups to evaluate the diversity of Citrobacter isolates from activated biomass of effluent treatment plants.

Environmental Genomics Unit, National Environmental Engineering Research Institute, CSIR, Nehru Marg, Nagpur 440 020, MS, India.
Bioresource Technology (Impact Factor: 5.04). 04/2008; 99(5):1189-95. DOI: 10.1016/j.biortech.2007.02.021
Source: PubMed

ABSTRACT The diversity of Citrobacter isolated from effluent treatment plants (ETPs) was studied using three different parameters. Thirty Citrobacter strains were isolated from different ETPs treating wastewaters generated at various industries. All the isolates were characterized based on biochemical tests, antibiotic assay/functional analysis, and phylogenetic analysis. Results demonstrated that the pattern of grouping varied based on the selected criteria for analysis. Species that clustered together by biochemical analysis were found to vary by functional and 16S rDNA analysis and vice versa. This suggests that multiple methods approach needs to be carried out to understand the microbial diversity. Bacteria in effluent treatment plants are exposed to diverse categories of pollutants. Salicylate is a key intermediate formed during biodegradation of several aromatic compounds, a scenario expected in ETPs. Hence, the Citrobacter isolates were screened for their capability to utilize salicylate. In future studies, these isolates can be incorporated in a bioremediation program.

0 Bookmarks
 · 
37 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The wastewater originating from simultaneous production of styrene and propylene oxide (SPO) is classified as highly polluted with chemical oxygen demand level in the range 5965 to 9137mgL(-1)-as well as highly toxic. The dilution factor providing for a 10 percent toxic effect of wastewater samples in a test with Paramecium caudatum was 8.0-9.5. Biological approach for pretreatment and detoxification of the wastewater under full-scale bioreactor conditions was investigated. The number of suspended microorganisms and the clean up efficiency were increased up to 5.5-6.58×10(8)CFUmL(-1) and 88 percent, respectively during the bioreactor's operation. Isolates in the Citrobacter, Burkholderia, Pseudomonas, and Paracoccus genera were dominant in the mature suspended, as well as the immobilized microbial community of the bioreactor. The most dominant representatives were tested for their ability to biodegrade the major components of the SPO wastewater and evidence of their role in the treatment process was demonstrated. The investigated pretreatment process allowed the wastewater to be detoxified for conventional treatment with activated sludge and was closely related to the maturation of the bioreactor's microbial community.
    Ecotoxicology and Environmental Safety 07/2014; 108C:195-202. · 2.20 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Identification of factors which can influence the natural attenuation process with available microbial genetic capacities can support the bioremediation which has been viewed as the safest procedure to combat with anthropogenic compounds in ecosystems. With the advent of molecular techniques, assimilatory capacity of an ecosystem can be defined with changing community dynamics, and if required, the essential genetic potential can be met through bioaugmentation. At the same time, intensification of microbial processes with nutrient balancing, expressing and enhancing the degradative capacities, could reduce the time frame of restoration of the ecosystem. The new concept of ecosystems biology has added greatly to conceptualize the networking of the evolving microbiota of the niche that helps in effective application of bioremediation tools to manage pollutants as additional carbon source.
    Applied biochemistry and biotechnology 12/2011; 166(4):903-24. · 1.94 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This article is a tribute to Carl R. Woese, a biophysicist turned evolutionary microbiologist who passed away on December 30, 2012. We focus on his life, achievements, the discovery of Archaea and contributions to the development of molecular phylogeny. Further, the authors share their views and the lessons learnt from Woese’s life with the microbiologists in India. We also emphasize the need for interdisciplinary collaboration and interaction for the progress and betterment of science.
    Indian Journal of Microbiology 09/2013; 53(3):247. · 0.46 Impact Factor

Atya Kapley