Effect of hyperbaric oxygen and vitamin C and E supplementation on biomarkers of oxidative stress in healthy men

Insititute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 28, D-70593, Stuttgart, Germany.
British Journal Of Nutrition (Impact Factor: 3.45). 11/2007; 98(4):826-33. DOI: 10.1017/S0007114507744380
Source: PubMed


The objectives of the present study were to evaluate the effect of normobaric and hyperbaric O2 (HBO) on plasma antioxidants and biomarkers of oxidative stress in plasma and urine and to investigate the effect of a 4-week vitamin C plus E supplementation on HBO-induced oxidative stress. Nineteen healthy men were exposed to HBO (100 % O2; 240 kPa) before and after 4 weeks' supplementation with 500 mg vitamin C plus 165 mg alpha-tocopherol equivalents. Exposure to 21 % O2 at 100 kPa served as intra-individual controls (control). Samples for the analysis of plasma antioxidants and oxidative stress biomarkers were collected before and immediately after each treatment. The present results showed that when compared with 'control', a single exposure to HBO resulted in a decrease of plasma vitamin C (P = 0.027) and an increase of lipid peroxides (P = 0.0008) and urinary 8-oxo-deoxyguanosine (8-oxodG) excretion (P = 0.006). Oxidative stress was not prevented by a 4-week supplementation with vitamins C and E. HBO-induced changes in plasma parameters correlated with basal antioxidant levels. The increase of urinary 8-oxodG after HBO plus supplementation correlated negatively with vitamin E intake (P = 0.023). We concluded that in healthy men HBO caused oxidative stress, which could not be prevented by dietary vitamin C plus E supplementation. The present data support the idea that HBO is a suitable model for oxidative stress in healthy volunteers.

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    • "Previously, oxidative stress has been implicated as a key transduction signal for various cytokines and hormones (Fisher-Wellman & Bloomer, 2009; Kalmar & Greensmith, 2009). Previous data demonstrate that a single exposure to HBO results in elevated lipid peroxides (Bader et al., 2007). Although there was an increase in TBARS post-exposure following HA in the present study, however, this did not reach statistical significance (Fig. 3). "
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    • "Consequently, antioxidants, such as vitamin C (VC), are widely used as therapeutic agents. However, the precise mechanism of antioxidant action is still unclear and the role of VC in oxidative stress is still controversial (Bader et al., 2007; Besaratinia et al., 2007). "
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