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Radiation-Induced Late Effects in Two Affected Individuals of the Lilo Radiation Accident

Bundeswehr Institute of Radiobiology, Neuherbergstr. 11, D-80937 Munich, Germany.
Radiation Research (Impact Factor: 2.45). 06/2007; 167(5):615-23. DOI: 10.1667/RR0774.1
Source: PubMed

ABSTRACT Radiation exposure leads to a risk for long-term deterministic and stochastic late effects. Two individuals exposed to protracted photon radiation in the radiological accident at the Lilo Military site in Georgia in 1997 received follow-up treatment and resection of several chronic radiation ulcers in the Bundeswehr Hospital Ulm, Germany, in 2003. Multi-parameter analysis revealed that spermatogenetic arrest and serum hormone levels in both patients had recovered compared to the status in 1997. However, we observed a persistence of altered T-cell ratios, increased ICAM1 and beta1-integrin expression, and aberrant bone marrow cells and lymphocytes with significantly increased translocations 6 years after the accident. This investigation thus identified altered end points still detectable years after the accident that suggest persistent genomic damage as well as epigenetic effects in these individuals, which may be associated with an elevated risk for the development of further late effects. Our observations further suggest the development of a chronic radiation syndrome and indicate follow-up parameters in radiation victims.

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    • "It has been known that T-lymphocytes mature in thymus which starts degrading soon after birth (Kusunoki et al. 1998). Decreased numbers of CD4? lymphocytes were also observed among Hiroshima and Nagasaki A-bomb survivors 42–46 years after acute exposure (Kusunoki et al. 1998, 1999), in residents of Techa riverside villages 53–56 years after exposure onset (Ovcharova 2006; Akleyev 2007); relation of CD4? lymphocytes to dose was revealed both in this study and the Japanese one (Kusunoki et al. 1998) and in gamma radiation-exposed individuals of the Lilo accident (Scherthan et al. 2007). Relative and absolute concentration of CD8? subset of T-lymphocytes was altered neither in this study nor in Japanese cohort (Kusunoki et al. 1998). "
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    • "It has been known, that T-lymphocytes differentiate in thymus which starts degrading soon after birth (Kusunoki et al. 1998). Decreased numbers of CD4+ lymphocytes was also observed among Hiroshima and Nagasaki A-bomb survivors 42-46 years after acute exposure (Kusunoki et al. 1998, 1999), in residents of Techa riverside villages 53-56 years after exposure onset (Ovcharova et al. 2006, Akleyev et al. 2007); relation of CD4+ lymphocytes to dose was revealed both in this study and the Japanese one (Kusunoki et al., 1998) and in gamma radiation-exposed individuals of the Lilo accident (Scherthan et al. 2007). Relative and absolute concentration of СD8+ subset of T-lymphocytes was altered neither in this study nor in Japanese cohort (Kusunoki et al., 1998). "
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    • "Slides were stored at −20 • C until use. Three-colour chromosome painting (FISH) with a combination of chromosome #1, #4 and #12 paint probes (MetaSystems, Altlussheim, Germany) was done as published [38] [39]. Briefly: The hybridization mix was heated to 72 • C for 5 min and then incubated at 37 • C for 10 min prior to applying the mixture to the denatured chromosomes. "
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