Mixing in 384-Well Plates: Issues, Measurements, and Solutions

Amgen Inc., Thousand Oaks, CA 91320, USA.
Assay and Drug Development Technologies (Impact Factor: 1.53). 05/2007; 5(2):265-75. DOI: 10.1089/adt.2006.042
Source: PubMed


Mixing in standard 384-well plates is different from mixing in 96-well formats. The aspect ratio of a typical well, the balance of surface tension and mass of the fluids, and the scale of diffusion all add to the increased difficulty in mixing fluids in higher-density plates. Here we examine two methods to measure mixing and some common techniques for mixing in 384-well plates. While conventional shaking can suffice, alternative methods can accelerate and improve the efficiency of mixing in 384-well plates.

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