Bacterial contamination and decontamination of toothbrushes after use.

Department of Community Dentistry, Jaipur Dental College, India.
The New York state dental journal 05/2007; 73(3):20-2.
Source: PubMed

ABSTRACT The purpose of this study was to determine the extent of bacterial contamination of toothbrushes after use and the efficacy of chlorhexidine and Listerine in decontaminating toothbrushes. The effectiveness of covering a toothbrush head with a plastic cap in preventing contamination was also evaluated. It was found that 70% of the used toothbrushes were heavily contaminated with different pathogenic microorganisms. Use of a cap leads to growth of opportunistic microorganisms like Pseudomonas aeruginosa, which may cause infection in the oral cavity. Overnight immersion of a toothbrush in chlorhexidine gluconate (0.2%) was found to be highly effective in preventing such microbial contamination.


Available from: Abhishek Mehta, Jan 09, 2015
1 Follower
  • Source
    [Show description] [Hide description]
    DESCRIPTION: Los instrumentos dentales y de higiene bucal usados frecuentemente para mantener una buena higiene bucal y prevenir la aparición de enfermedades pueden contribuir a la transmisión de microorganismos patógenos si no se usan correctamente tal es el caso del cepillo dental. Esta investigación evaluó la presencia de Enterobacterias en cerdas de cepillos dentales y exudados faríngeos de los estudiantes del 4to año de la Escuela de Bioanálisis de la Universidad de Carabobo. El estudio fue correlacional, de campo y de corte transversal. Se evaluaron 90 estudiantes divididos en grupo A (casos) y grupo B (control), determinándose la presencia de Enterobacterias en cerdas de cepillos dentales de ambos grupos, observándose una mayor positividad en los cepillos del grupo A (76%), en comparación al grupo B (40%). También se determinó la presencia de Enterobacterias en exudados faríngeos de ambos grupos, en el que se encontró una mayor prevalencia de Enterobacterias en el grupo A (51%) con respecto al grupo B (36%). Entre los principales factores de riesgo que contribuyeron a la contaminación de los cepillos dentales estuvieron el guardado del cepillo dental en el cuarto de baño, el contacto con otros cepillos dentales durante su guardado y el tiempo prolongado de uso. Las Enterobacterias más aisladas en esta investigación fueron Klebsiella pneumoniae y Escherichia coli. Se concluye que existe una asociación estadísticamente significativa (p < 0.05) entre la presencia de Enterobacterias en las cerdas de los cepillos dentales y la colonización de estas bacterias en la faringe de los estudiantes. Palabras clave: Cepillo dental, Enterobacterias, Exudado Faríngeo. Trabajo Presentado en el marco de VIII Congreso Nacional y II internacional de Investigación de la Universidad de Carabobo.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To assess and compare the efficacy of 3% garlic extract, 0.2% tea tree oil, 0.2% chlorhexidine, 0.05% cetylpyridinium chloride, and ultravoilet (UV) toothbrush sanitizing device as toothbrush disinfectants against Streptococcus mutans. A double blind randomized controlled parallel study was done on 210 dental students. The subjects were divided into one control group using distilled water and five study groups representing 0.2% tea tree oil, 3% garlic extract, 0.2% chlorhexidine gluconate, 0.05% cetylpyridinium chloride and UV toothbrush sanitizing device. Participants were provided with new toothbrushes and toothpastes for both baseline and intervention phases. The toothbrushes were collected after two weeks for microbial analysis in both phases. The data were analysed and compared using appropriate statistical analysis. On comparing pre- and post-intervention, S. mutans colony counts, a highly significant (P < 0.001) difference was observed in all the groups. Differences of 77.74 colony forming units (CFU) in tea tree oil group, 102.87 CFU in garlic group, 68.13 CFU in chlorhexidine group, 82.47 CFU in cetylpyridinium group and 42.67 CFU in UV toothbrush sanitizer group were observed. Garlic group showed the highest reduction (100%) whereas UV toothbrush sanitizer group showed the least reduction (47.4%) in S. mutans colonies. The antimicrobial agents used in this study effectively reduced the S. mutans counts and hence can be considered as toothbrush disinfectants to prevent dental caries. The 3% garlic was the most effective among the antimicrobial agents.
    10/2014; 5(4):183-9. DOI:10.4103/0975-962X.144718
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: PURPOSE: This study evaluated the rate of microbial contamination of toothbrushes used by students and established a protocol for the spraying of 0.12% chlorhexidine to decrease the bacterial presence on the bristles of the brushes. METHODS: We performed cultures on selective and nonselective media from toothbrushes used by Dentistry students from State University of West Paraná (Unioeste/PR) to identify and quantify the number of total and specific microorganisms. For the chlorhexidine test, a crossover and double-blind study was conducted, with a random selection of volunteers. Each experimental phase consisted of fourteen days of brushing and an interval of seven days between treatments. For the test, thirty volunteers, divided into three groups of 10, using new toothbrushes, sprayed with either water or 0.12% chlorhexidine gluconate at different intervals (once or three times daily) after brushing. For statistical analysis, we used main effects ANOVA, and a P-value<0.05 was considered significant. RESULTS: There was microbial growth on 91% of the used toothbrushes, with 81.3% showing growth of Streptococcus. In 56.3% of the brushes, Staphylococcus and Enterobacteriaceae developed. The use of the chlorhexidine spray only three times per day was significantly more effective than water. CONCLUSION: Even in a group that knows the ideal conditions for the storage of toothbrushes, much microbial contamination still exists on the toothbrushes. A lower bacterial load can be obtained from the simple and inexpensive act of spraying chlorhexidine on the toothbrush after every brushing of the day.
    12/2011; 27(3):213-217. DOI:10.1590/S1980-65232012000300007