Article

The IGF-I splice variant MGF increases progenitor cells in ALS, dystrophic, and normal muscle.

Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, University College London, UK.
FEBS Letters (impact factor: 3.54). 07/2007; 581(14):2727-32. DOI:10.1016/j.febslet.2007.05.030 pp.2727-32
Source: PubMed

ABSTRACT The effects of muscle splice variants of insulin-like growth factor I (IGF-I) on proliferation and differentiation were studied in human primary muscle cell cultures from healthy subjects as well as from muscular dystrophy and ALS patients. Although the initial numbers of mononucleated progenitor cells expressing desmin were lower in diseased muscle, the E domain peptide of IGF-IEc (MGF) significantly increased the numbers of progenitor cells in healthy and diseased muscle. IGF-I significantly enhances myogenic differentiation whereas MGF E peptide blocks this pathway, resulting in an increased progenitor (stem) cell pool and thus potentially facilitating repair and maintenance of this postmitotic tissue.

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Keywords

ALS patients
 
desmin
 
diseased muscle
 
E domain peptide
 
healthy
 
healthy subjects
 
human primary muscle cell cultures
 
IGF-I
 
IGF-IEc
 
increased progenitor
 
initial numbers
 
insulin-like growth factor
 
MGF
 
MGF E peptide blocks
 
mononucleated progenitor cells
 
muscle splice variants
 
muscular dystrophy
 
postmitotic tissue
 
progenitor cells
 
proliferation
 

Kenan Ates