[Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction].
ABSTRACT To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).
Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.
The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.
Single cell fluorescent PCR is a stable and reliable approach for the PGD.