Article

Reduced secretion and expression of gelatinase profile in Toxoplasma gondii-infected human monocytic cells.

Laboratoire de Biochimie et Biologie Moléculaire, CNRS UMR 6198, IFR 53, UFR Médecine, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095 Reims cedex, France.
Biochemical and Biophysical Research Communications (impact factor: 2.48). 08/2007; 359(2):298-303. DOI:10.1016/j.bbrc.2007.05.089 pp.298-303
Source: PubMed

ABSTRACT The apicomplexan Toxoplasma gondii, an obligate intracellular parasite, can infect humans and a wide range of vertebrates. Following oral infection, the parasite invades tissues by crossing non-permissive biological barriers such as the placenta or the blood-brain barrier. But the molecular mechanisms underlying migration of T. gondii remain poorly characterized. The crossing of various basal membranes and infiltration into the extracellular matrix by T. gondii could involve matrix metalloproteinases (MMPs). We demonstrated a decrease in proMMP-2 and proMMP-9 secretion by THP-1 cells at 24 and 48h post invasion with regulation at the mRNA level throughout infection. This down regulation was associated with a decrease in TIMP-2 secretion and an inhibition of its expression. Moreover, results showed an activation of MT1-MMP; its expression was regulated after 6, 24, and 48h.

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Keywords

48h post invasion
 
activation
 
apicomplexan Toxoplasma gondii
 
blood-brain barrier
 
decrease
 
infect humans
 
inhibition
 
matrix metalloproteinases
 
molecular mechanisms
 
non-permissive biological barriers
 
obligate intracellular parasite
 
oral infection
 
proMMP-9 secretion
 
T. gondii
 
THP-1 cells
 
various basal membranes
 
wide range