Article

Processing MALDI Mass Spectra to Improve Mass Spectral Direct Tissue Analysis.

Protein Discovery, Inc., 418 S. Gay Street-Suite 203, Knoxville, TN 37902, United States.
International Journal of Mass Spectrometry (impact factor: 2.55). 03/2007; 260(2-3):212-221. DOI:10.1016/j.ijms.2006.10.005 pp.212-221
Source: PubMed

ABSTRACT Profiling and imaging biological specimens using MALDI mass spectrometry has significant potential to contribute to our understanding and diagnosis of disease. The technique is efficient and high-throughput providing a wealth of data about the biological state of the sample from a very simple and direct experiment. However, in order for these techniques to be put to use for clinical purposes, the approaches used to process and analyze the data must improve. This study examines some of the existing tools to baseline subtract, normalize, align, and remove spectral noise for MALDI data, comparing the advantages of each. A preferred workflow is presented that can be easily implemented for data in ASCII format. The advantages of using such an approach are discussed for both molecular profiling and imaging mass spectrometry.

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Keywords

advantages
 
ASCII format
 
clinical purposes
 
direct experiment
 
imaging biological specimens
 
imaging mass spectrometry
 
MALDI data
 
MALDI mass spectrometry
 
molecular profiling
 
preferred workflow
 
spectral noise
 
study examines
 
techniques