Velazquez OCAngiogenesis and vasculogenesis: inducing the growth of new blood vessels and wound healing by stimulation of bone marrow-derived progenitor cell mobilization and homing. J Vasc Surg 45(Suppl A):A39-A47

Department of Surgery, University of Pennsylvania Medical Center, Philadelphia, PA 19104-4283, USA.
Journal of Vascular Surgery (Impact Factor: 3.02). 07/2007; 45 Suppl A(6):A39-47. DOI: 10.1016/j.jvs.2007.02.068
Source: PubMed


During embryonic development, the vasculature is among the first organs to form and is in charge of maintaining metabolic homeostasis by supplying oxygen and nutrients and removing waste products. As one would expect, blood vessels are critical not only for organ growth in the embryo but also for repair of wounded tissue in the adult. An imbalance in angiogenesis (a time-honored term that globally refers to the growth of new blood vessels) contributes to the pathogenesis of numerous malignant, inflammatory, ischemic, infectious, immune, and wound-healing disorders. This review focuses on the central role of the growth of new blood vessels in ischemic and diabetic wound healing and defines the most current nomenclature that describes the neovascularization process in wounds. There are now two well-defined, distinct, yet interrelated processes for the formation of postnatal new blood vessels, angiogenesis, and vasculogenesis. Reviewed are recent new data on vasculogenesis that promise to advance the field of wound healing.

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    • "The presence of the initial hernia and the high risk of recurrence are indicative of a breakdown in the normal wound healing process. One central component of the wound healing process is the restoration of the metabolic capacity of damaged tissue through angiogenesis and vasculogenesis, two separate but related processes [2]. "
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    ABSTRACT: Introduction: In adipose tissue healing, angiogenesis is stimulated by adipose-derived stromal stem cells (ASCs). Ventral hernia repair (VHR) patients are at high risk for wound infections. We hypothesize that ASCs from VHR patients are less vasculogenic than ASCs from healthy controls. Methods: ASCs were harvested from the subcutaneous fat of patients undergoing VHR by the component separation technique and from matched abdominoplasty patients. RNA and protein were harvested on culture days 0 and 3. Both groups of ASCs were subjected to hypoxic conditions for 12 and 24 hours. RNA was analyzed using qRT-PCR, and protein was used for western blotting. ASCs were also grown in Matrigel under hypoxic conditions and assayed for tubule formation after 24 hours. Results: Hernia patient ASCs demonstrated decreased levels of VEGF-A protein and vasculogenic RNA at 3 days of growth in differentiation media. There were also decreases in VEGF-A protein and vasculogenic RNA after growth in hypoxic conditions compared to control ASCs. After 24 hours in hypoxia, VHR ASCs formed fewer tubules in Matrigel than in control patient ASCs. Conclusion: ASCs derived from VHR patients appear to express fewer vasculogenic markers and form fewer tubules in Matrigel than ASCs from abdominoplasty patients, suggesting decreased vasculogenic activity.
    03/2014; 2014(6):983715. DOI:10.1155/2014/983715
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    • "While angiogenesis occurs throughout fetal growth and development, in adults it is limited to the endometrium during the menstrual cycle, the ovary during formation of the corpus luteum and in pathological states including wound healing, diabetic retinopathy, tumor growth, and endometriosis [40–44]. This process requires new endothelial cells to sprout and then recruit pericytes to form capillaries or smooth muscle cells to form larger vessels [43, 45]. Subsequent steps include focal ECM degradation, endothelial cell proliferation and migration, organization of endothelial cells into capillary networks and lumen formation [46–50]. "
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    ABSTRACT: Tissue factor (TF), is a cellular receptor that binds the factor VII/VIIa to initiate the blood coagulation cascade. In addition to its role as the initiator of the hemostatic cascade, TF is known to be involved in angiogenesis via intracellular signaling that utilizes the protease activated receptor-2 (PAR-2). We now review the physiologic expression of TF in the endometrium and its altered expression in multiple cell types derived from eutopic and ectopic endometrium from women with endometriosis compared with normal endometrium. Our findings suggest that TF might be an ideal target for therapeutic intervention in endometriosis. We have employed a novel immunoconjugate molecule known as Icon and were able to eradicate endometrial lesions in a mouse model of endometriosis without affecting fertility. These findings have major implications for potential treatment in humans.
    07/2012; 2012:306830. DOI:10.6064/2012/306830
    • "Most chronic wounds are hypothesized to have a local environment with reduced oxygen levels.[8] Indeed, hyperoxia recruits bone marrow-derived progenitor cells into diabetic and ischaemic wounds.[9] The resident fibroblasts in chronic wounds may be phenotypically senescent and show decreased responsiveness to TGF and PDGF.[10] "
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    ABSTRACT: Chronic wounds continue to be a major challenge for the medical profession, and plastic surgeons are frequently called in to help in the management of such wounds. Apart from the obvious morbidity to the patient, these problem wounds can be a major drain on the already scarce hospital resources. Sometimes, these chronic wounds can be more taxing than the underlying disease itself. Although many newer methods are available to handle such situations, the role of stem cells in the management of such wounds is an exciting area that needs to be explored further. A review of literature has been done regarding the role of stem cells in the management of chronic wounds. The abnormal pathology in such wounds is discussed and the possible role of stem cells for optimal healing in such cases would be detailed.
    Indian Journal of Plastic Surgery 05/2012; 45(2):237-43. DOI:10.4103/0970-0358.101286
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