Anti-Mullerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization
ABSTRACT To determine whether the anti-Müllerian hormone (AMH) level in follicular fluid is associated with success in oocyte fertilization during in vitro fertilization (IVF) cycles.
Tokyo Medical University hospital.
Thirty-one women undergoing IVF cycles.
The women were divided into two groups according to the success or failure of fertilization. Follicular fluid samples were obtained from a single follicle in each patient.
Assessment of values for AMH, inhibin B, and estradiol from follicular fluid and serum on the day of oocyte retrieval during IVF treatment.
The follicular fluid AMH levels from fertilized patients (group 1) were 3.42 times higher than from nonfertilized (group 2). There was no correlation between the ratio of the high quality grade embryos and either serum AMH or inhibin B levels. There was no statistically significant correlation between serum AMH and the number of oocytes. However, there was a strong correlation between levels of serum estradiol, inhibin B, and the number of oocytes.
Oocytes are more likely to be fertilized when follicles are able to make high concentrations of AMH in the follicular fluid. Thus, AMH could be a prediction marker for fertilization.
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ABSTRACT: This study investigated anti-Mullerian hormone (AMH) expression and secretion from cumulus granulosa cells (GC) and steroidogenesis in follicular fluids (FF) with relation to oocyte maturational stages and fertilization capacity in large preovulatory follicles. This prospective study included 53 ovulatory women undergoing intracytoplasmic sperm injection. FF and cumulus GC from 140 large preovulatory follicles were individually obtained during oocyte retrieval. Main outcome measures were oocyte maturation, fertilization and embryo quality. FF were assayed for AMH, progesterone, 17 beta-oestradiol and testosterone. Cumulus GC were assayed for AMH mRNA expression. AMH mRNA expression and secretion in cumulus GC in preovulatory follicles containing germinal-vesicle (GV) and metaphase-I (MI) oocytes were significantly higher than follicles containing MII oocytes (P < 0.01 and P < 0.0001, respectively). In addition, FF AMH concentrations from atretic oocytes were significantly higher than from MII oocytes. No correlation was found between AMH expression and secretion to fertilization or embryo quality. FF of MI and GV oocytes had higher concentrations of testosterone and lower progesterone/oestradiol ratios than MII oocytes, and FF of atretic oocytes contained higher testosterone concentrations than FF of MII oocytes. AMH is highly expressed in and secreted from cumulus GC of preovulatory follicles containing premature and atretic oocytes.Reproductive biomedicine online 01/2012; 24(5):540-6. DOI:10.1016/j.rbmo.2012.01.023 · 2.98 Impact Factor
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ABSTRACT: A limitation to our ability to distinguish between developmentally competent and incompetent eggs is our still only partial knowledge of the critical features that are needed to make a good egg and when during oogenesis these specific characteristics are acquired. The main objective of this review is to summarize the results of areas of investigation that are contributing to our still inadequate understanding of the molecular aspects of making developmentally competent eggs. For each area discussed, a systematic search was made using PubMed. The search was without temporal limits but mainly yielded publications between 1982-1999 (23%) and 2000-2011 (77%). Taking an oocyte-centred view, we describe throughout folliculogenesis: (i) the factors that regulate oocyte growth; (ii) the role of oocyte-cumulus cell dialogue; (iii) the epigenetic organization of the oocyte genome and (iv) the storage and regulation of maternal RNAs. The multifaceted complex of factors involved in oocyte growth constitutes the backbone on which oocyte developmental competence is built up. Operating behind the expression of these factors is a specific epigenetic signature established during oogenesis, but our knowledge is only approximate and major efforts will be required for more accurate analyses at specific gene loci. The growing research on small silencing RNAs during oogenesis and early oocyte development is revealing these molecules' critical role in mRNA degradation. Our next challenge will be to dissect the complex interactions among the different molecular players identified and to establish the presence of functional links among these factors.Human Reproduction Update 03/2011; 17(4):525-40. DOI:10.1093/humupd/dmr009 · 8.66 Impact Factor
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ABSTRACT: V izolski porodnišnici je v obdobju od julija 2003 do decembra 2006 214 (10 %) porodnic rodilo s pomočjo EPA. V skupini porodnic z EPA v primerjavi s kontrolno skupino je bila statistično značilno večja povprečna starost porodnic (30,5 proti 28,7 let; p < 0,0005), podalj- šano trajanje poroda (278 proti 222 minut; p < 0,0005), večje število porodov, pospešenih z infuzijo oksitocina (93,4 % proti 72,9 %; p < 0,0001) ter večje število dokončanja poroda z izhodnim posegom (vakuumsko ekstrakcijo 14 % proti 1,9 %; p < 0,0001). Izid poroda s carskim rezom je bil primerljiv v obeh skupinah. Uporaba EPA kljub večjemu številu izho- dnih posegov in daljšim porodom ni vplivala na perinatalne izide in ni pomembnih razlik v oceni vitalnosti novorojenčka po Apgarjevi. Povprečna bolečina, ocenjena po lestvici VAS, je bila največja pred uporabo EPA (VAS 7), najmanjša med porodom (VAS 1,5) in nekoliko večja med iztisom (VAS 2,7). Večina porodnic je bila z uporabo EPA zadovoljnih, 92 % porodnic je analgezijo dan po porodu ocenilo kot dobro ali zelo dobro. Zaključki Uporaba EPA je primerna in učinkovita metoda za lajšanje porodne bolečine. Zadovoljstvo porodnic s tem načinom lajšanja porodne bolečine je veliko. Uporaba EPA kljub večjem številu izhodnih posegov in daljšim porodom ni vplivala na morebitne slabše perinatalne izide. Abstract Background The study presents the experience with epidural analgesia (EPA) for pain relief in Izola General Hospital from 2003 to 2006, the differences of labour between epidural analgesia and without it and the parturients' satisfaction.