Anti-Mullerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization

Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, Japan.
Fertility and sterility (Impact Factor: 4.59). 04/2008; 89(3):586-91. DOI: 10.1016/j.fertnstert.2007.03.080
Source: PubMed


To determine whether the anti-Müllerian hormone (AMH) level in follicular fluid is associated with success in oocyte fertilization during in vitro fertilization (IVF) cycles.
Retrospective study.
Tokyo Medical University hospital.
Thirty-one women undergoing IVF cycles.
The women were divided into two groups according to the success or failure of fertilization. Follicular fluid samples were obtained from a single follicle in each patient.
Assessment of values for AMH, inhibin B, and estradiol from follicular fluid and serum on the day of oocyte retrieval during IVF treatment.
The follicular fluid AMH levels from fertilized patients (group 1) were 3.42 times higher than from nonfertilized (group 2). There was no correlation between the ratio of the high quality grade embryos and either serum AMH or inhibin B levels. There was no statistically significant correlation between serum AMH and the number of oocytes. However, there was a strong correlation between levels of serum estradiol, inhibin B, and the number of oocytes.
Oocytes are more likely to be fertilized when follicles are able to make high concentrations of AMH in the follicular fluid. Thus, AMH could be a prediction marker for fertilization.

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    • "Fanchin et al. (14) demonstrated that FF AMH levels of the preovulatory follicle are positively associated with embryo implantation. Takahashi et al. (16) showed that oocytes are more likely to be fertilized when follicles have high concentrations of AMH. However, there have been very few studies on the direct relationship between FF AMH levels and quality of oocyte and embryo and this issue require further study. "
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    ABSTRACT: This prospective study investigated the relationship between anti-Müllerian hormone (AMH) level in the follicular fluid (FF) and the quality of the oocyte and embryo. A total of 65 FF samples from 54 women were included in this study. FF was collected from the largest preovulatory follicle sized≥20 mm of mean diameter from each ovary. Samples were divided into 3 groups according to the FF AMH levels: below the 33th percentile (low group, FF AMH<2.1 ng/mL, n=21), between the 33th and the 67th percentile (intermediate group, FF AMH=2.1-3.6 ng/mL, n=22), and above the 67th percentile (high group, FF AMH>3.6 ng/mL, n=22). The quality of the ensuing oocytes and embryos was evaluated by fertilization rate and embryo score. FF AMH levels correlated positively with the matched embryo score on day 3 after fertilization (r=0.331, P=0.015). The normal fertilization rate was significantly lower in the low group than in the intermediate group (61.9% vs. 95.5% vs. 77.3%, respectively, P=0.028). Our results suggest that the FF AMH level could be a predictor of the ensuing oocyte and embryo quality. Graphical Abstract
    Journal of Korean Medical Science 09/2014; 29(9):1266-70. DOI:10.3346/jkms.2014.29.9.1266 · 1.27 Impact Factor
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    • "These results are consistent with the results of Fanchin et al. (2007). On the other hand, a retrospective study by Takahashi et al. (2008) found a positive relationship between FF AMH and fertilization rate. Fertilization rate depends on factors other than oocyte quality (e.g. "
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    ABSTRACT: This study investigated anti-Mullerian hormone (AMH) expression and secretion from cumulus granulosa cells (GC) and steroidogenesis in follicular fluids (FF) with relation to oocyte maturational stages and fertilization capacity in large preovulatory follicles. This prospective study included 53 ovulatory women undergoing intracytoplasmic sperm injection. FF and cumulus GC from 140 large preovulatory follicles were individually obtained during oocyte retrieval. Main outcome measures were oocyte maturation, fertilization and embryo quality. FF were assayed for AMH, progesterone, 17 beta-oestradiol and testosterone. Cumulus GC were assayed for AMH mRNA expression. AMH mRNA expression and secretion in cumulus GC in preovulatory follicles containing germinal-vesicle (GV) and metaphase-I (MI) oocytes were significantly higher than follicles containing MII oocytes (P < 0.01 and P < 0.0001, respectively). In addition, FF AMH concentrations from atretic oocytes were significantly higher than from MII oocytes. No correlation was found between AMH expression and secretion to fertilization or embryo quality. FF of MI and GV oocytes had higher concentrations of testosterone and lower progesterone/oestradiol ratios than MII oocytes, and FF of atretic oocytes contained higher testosterone concentrations than FF of MII oocytes. AMH is highly expressed in and secreted from cumulus GC of preovulatory follicles containing premature and atretic oocytes.
    Reproductive biomedicine online 01/2012; 24(5):540-6. DOI:10.1016/j.rbmo.2012.01.023 · 3.02 Impact Factor
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    • "Irez et al. have recently demonstrated the significant association between basal AMH serum concentration with the total number of oocytes and AFC, inhibin-B and FSH levels, oocyte quality and embryo development in intracytoplasmic sperm injection (ICSI) cycles. Also, the authors observed that early embryo cleavage rate was significantly higher among patients with AMH serum levels between 1.40 ng/mL and 4.83 ng/mL [74], supporting the hypothetical association of AMH with implantation rates [75–77]. Likewise, Arabzadeh et al. have recently demonstrated positive correlation between serum basal AMH levels and the number of oocytes retrieved and the percentage of mature oocytes, but significance was also not reached in correlation between the marker and implantation rate [78]. "
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    ABSTRACT: The current trends to postpone motherhood and the increase in demand for assistance in reproductive medicine highlight the need for seeking guidelines for the establishment of individualized treatment protocols. Currently available ovarian reserve tests do not provide sufficient evidence to be solely considered ideal, but they may occupy important place in initial counseling, predicting unsatisfactory results that could be improved by individualized induction schemes and reducing excessive psychological and financial burdens, and adverse effects. In this paper, we revise the role of hormonal basal and dynamic tests, as well as ultrasonographic markers, as ovarian reserve markers, in order to provide embasement for propaedeutic strategies and their interpretation in order to have reproductive success.
    ISRN obstetrics and gynecology 01/2012; 2012:576385. DOI:10.5402/2012/576385
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