Identification of Proteolytic Cleavage Sites by Quantitative Proteomics

Department of Biochemistry, Purdue University, ウェストラファイエット, Indiana, United States
Journal of Proteome Research (Impact Factor: 4.25). 08/2007; 6(7):2850-8. DOI: 10.1021/pr0701052
Source: PubMed


The identification of natural substrates and their cleavage sites is pivotal to defining proteolytic pathways. Here we report a novel strategy for the identification of the signature of proteolytic cleavage events based on quantitative proteomics. Lysine residues in proteins are blocked by guanidination so that free N-terminals can be labeled with amine-specific iTRAQ reagents. The quantitative nature of iTRAQ reagents allows us to distinguish N-terminals newly formed by proteolytic treatment (neoepitopes) from original N-terminals in proteins. Proteins are digested with trypsin and analyzed using MALDI-TOF/TOF mass spectrometry. Peptides labeled with iTRAQ reagents are distinguished from other peptides by exhibiting intense signature ions in tandem mass spectrometry analysis. A corresponding data acquisition strategy was developed to specifically analyze iTRAQ tagged N-terminal peptides. To validate the procedure, we examined a set of recombinant Escherichia coli proteins that have predicted caspase-3 cleavage motifs. The protein mixture was treated with active or inactive caspase-3 and subsequently labeled with two different iTRAQ reagents. Mass spectrometric analysis located 10 cleavage sites, all corresponding to caspase-3 consensus. Spiking caspase-cleaved substrate into a human cell lysate demonstrated the high sensitivity of the procedure. Moreover, we were able to identify proteolytic cleavage products associated with the induction of cell-free apoptosis. Together, these data reveal a novel application for iTRAQ technology for the detection of proteolytic substrates.

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    • "Most of the studies utilized this modification on peptide levels. [22] [23] To consider trypsin-cleaved homoarginine-terminated peptides for our enriched N-terminal peptides search, we performed a trypsin digestion study of two different homoarginine-modified proteins, ubiquitin and β-casein. At first, a small protein, bovine ubiquitin (~8.6 kDa), was used for this study. "
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    ABSTRACT: Proteolytic cleavages generate active precursor proteins by creating new N-termini in the proteins. A number of strategies have recently been published regarding the enrichment of original or newly formed N-terminal peptides using guanidination of lysine residues and amine-reactive reagents. For effective enrichment of N-terminal peptides, the efficiency of trypsin proteolysis on homoarginine (guanidinated) modified proteins must be understood and simple and versatile solid-phase N-terminal capture strategies should be developed. We present here a mass spectrometry (MS)-based study to evaluate and optimize the trypsin proteolysis on a guanidinated-modified protein. Trypsin proteolysis was studied using different amounts of trypsin to modified protein ratios. To capture the original N-termini, after guanidination of proteins, original N-termini were acetylated and the proteins were digested with trypsin. The newly formed N-terminal tryptic peptides were captured with a new amine reactive acid-cleavable solid-phase reagent. The original N-terminal peptides were then collected from the supernatant of the solution. We demonstrated a detailed study of the efficiency of enzyme trypsin on homoarginine-modified proteins. We observed that the rate of hydrolysis of homoarginine residues compared to their lysine/arginine counterparts were slower but generally cleaved after an overnight digestion period depending on the protein to protease concentration ratios. Selectivity of the solid-phase N-terminal reagent was studied by enrichment of original N-terminal peptides from two standard proteins, ubiquitin and RNaseS. We found enzyme trypsin is active in the guanidinated form of the protein depending on the enzyme to protein concentrations, time and the proximity of arginine residues in the sequence. The novel solid-phase capture reagent also successfully enriched N-terminal peptides from the standard protein mixtures. We believe this trypsin proteolysis study on homoarginine-modified proteins and our simple and versatile solid-phase capture strategy could be very useful for enrichment and sequence determination of proteins N-termini by MS. Copyright © 2014 John Wiley & Sons, Ltd.
    Rapid Communications in Mass Spectrometry 03/2014; 28(6):635-44. DOI:10.1002/rcm.6820 · 2.25 Impact Factor
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    • "non-gel-based methods such as combined fractional diagonal chromatography (COFRADIC; Gevaert et al, 2003), proteomic identification of protease cleavage sites (PICS; Schilling and Overall, 2008), terminal amine isotopic labelling of substrates (TAILS; Kleifeld et al, 2010), ICAT and iTRAQ variations (Tam et al, 2004; Dean and Overall, 2007; Enoksson et al, 2007), or genetically engineered enzyme use (Mahrus et al, 2008) were found to be superior, leading to identification of a great number of protease substrates, including those of caspases, granzyme B, and various MMPs (Van Damme et al, 2005, 2009; Mahrus et al, 2008; Morrison et al, 2009). These methods, in addition to identifying cleavage sites within protein substrates, enable determination of protease specificities , especially when used on the larger peptidic fragments that are generated from proteins by trypsin, endo GluC or some other protease cleavage, or on total cellular lysates. "
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    ABSTRACT: Protease research has undergone a major expansion in the last decade, largely due to the extremely rapid development of new technologies, such as quantitative proteomics and in-vivo imaging, as well as an extensive use of in-vivo models. These have led to identification of physiological substrates and resulted in a paradigm shift from the concept of proteases as protein-degrading enzymes to proteases as key signalling molecules. However, we are still at the beginning of an understanding of protease signalling pathways. We have only identified a minor subset of true physiological substrates for a limited number of proteases, and their physiological regulation is still not well understood. Similarly, links with other signalling systems are not well established. Herein, we will highlight current challenges in protease research.
    The EMBO Journal 02/2012; 31(7):1630-43. DOI:10.1038/emboj.2012.42 · 10.43 Impact Factor
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    • "Selective guanidination protection of ε-amines (Enoksson et al. 2007, Timmer et al. 2007) allows for modification of free N-terminal α-amines with sulfosuccinimidyl "
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    ABSTRACT: Proteolysis is an irreversible post-translational modification that regulates many intra- and intercellular processes, including essential go/no-go decisions during cell proliferation, development and cell death. Hundreds of protease-coding genes have been identified in plants, but few have been linked to specific substrates. Conversely, proteolytic processes are frequently observed in plant biology but rarely have they been ascribed to specific proteases. In mammalian systems, unbiased system-wide proteomics analyses of protease activities have recently been tremendously successful in the identification of protease substrate repertoires, also known as substrate degradomes. Knowledge of the substrate degradome is key to understand the role of proteases in vivo. Quantitative shotgun proteomic studies have been successful in identifying protease substrates, but while simple to perform they are biased toward abundant proteins and do not reveal precise cleavage sites. Current degradomics techniques overcome these limitations by focusing on the information-rich amino- and carboxy-terminal peptides of the original mature proteins and the protease-generated neo-termini. Targeted quantitative analysis of protein termini identifies precise cleavage sites in protease substrates with exquisite sensitivity and dynamic range in in vitro and in vivo systems. This review provides an overview of state-of-the-art methods for enrichment of protein terminal peptides, and their application to protease research. These emerging degradomics techniques promise to clarify the elusive biological roles of proteases and proteolysis in plants.
    Physiologia Plantarum 10/2011; 145(1):5-17. DOI:10.1111/j.1399-3054.2011.01536.x · 3.14 Impact Factor
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