Karnani, N., Taylor, C., Malhotra, A. & Dutta, A. Pan-S replication patterns and chromosomal domains defined by genome-tiling arrays of ENCODE genomic areas. Genome Res. 17, 865-876

Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, USA.
Genome Research (Impact Factor: 14.63). 07/2007; 17(6):865-76. DOI: 10.1101/gr.5427007
Source: PubMed


In eukaryotes, accurate control of replication time is required for the efficient completion of S phase and maintenance of genome stability. We present a high-resolution genome-tiling array-based profile of replication timing for approximately 1% of the human genome studied by The ENCODE Project Consortium. Twenty percent of the investigated segments replicate asynchronously (pan-S). These areas are rich in genes and CpG islands, features they share with early-replicating loci. Interphase FISH showed that pan-S replication is a consequence of interallelic variation in replication time and is not an artifact derived from a specific cell cycle synchronization method or from aneuploidy. The interallelic variation in replication time is likely due to interallelic variation in chromatin environment, because while the early- or late-replicating areas were exclusively enriched in activating or repressing histone modifications, respectively, the pan-S areas had both types of histone modification. The replication profile of the chromosomes identified contiguous chromosomal segments of hundreds of kilobases separated by smaller segments where the replication time underwent an acute transition. Close examination of one such segment demonstrated that the delay of replication time was accompanied by a decrease in level of gene expression and appearance of repressive chromatin marks, suggesting that the transition segments are boundary elements separating chromosomal domains with different chromatin environments.

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Available from: Ankit Malhotra, Apr 16, 2014
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    • "All loci analyzed showed a single 1-hr peak interval, during which the BrdU incorporation was greatest (Fig. 2B). It is known that ADH5 and the gamma-globin gene replicate in early and late S phase, respectively [30], [31]. When cumulative BrdU incorporation was correlated with specific test loci in fractions covering the S phase progression, we found that ADH5 and gamma-globin loci replicated at early and late S phase, respectively, as expected (Fig. 2C). "
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    • "Changes in replication timing during development (see below) can strongly influence the genome-wide alignment of replication timing to isochores (Hiratani et al. 2010; Ryba et al. 2010). Moreover, homologous loci can replicate at different times in the same cells (Karnani et al. 2007; Farkash-Amar et al. 2008; Hansen et al. 2010). Isochores that exhibit the most extreme of the above-mentioned sequence compositions tend to replicate at the same time in all tissues, whereas regions with intermediate or mixed sequence features are more prone to change replication timing during development (Fig. 3, top), suggesting that sequence composition has some indirect influence on replication timing. "
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