Article

Analysis of overrepresented motifs in human core promoters reveals dual regulatory roles of YY1

Bioinformatics Program, Boston University, Boston, Massachusetts 02215, USA.
Genome Research (Impact Factor: 13.85). 07/2007; 17(6):798-806. DOI: 10.1101/gr.5754707
Source: PubMed

ABSTRACT A set of 723 high-quality human core promoter sequences were compiled and analyzed for overrepresented motifs. Beside the two well-characterized core promoter motifs (TATA and Inr), several known motifs (YY1, Sp1, NRF-1, NRF-2, CAAT, and CREB) and one potentially new motif (motif8) were found. Interestingly, YY1 and motif8 mostly reside immediately downstream from the TSS. In particular, the YY1 motif occurs primarily in genes with 5'-UTRs shorter than 40 base pairs (bp) and its locations coincide with the translation start site. We verified that the YY1 motif is bound by YY1 in vitro. We then performed detailed analysis on YY1 chromatin immunoprecipitation data with a whole-genome human promoter microarray (ChIP-chip) and revealed that the thus identified promoters in HeLa cells were highly enriched with the YY1 motif. Moreover, the motif overlapped with the translation start sites on the plus strand of a group of genes, many with short 5'-UTRs, and with the transcription start sites on the minus strand of another distinct group of genes; together, the two groups of genes accounted for the majority of the YY1-bound promoters in the ChIP-chip data. Furthermore, the first group of genes was highly enriched in the functional categories of ribosomal proteins and nuclear-encoded mitochondria proteins. We suggest that the YY1 motif plays a dual role in both transcription and translation initiation of these genes. We also discuss the evolutionary advantages of housing a transcriptional element inside the transcript in terms of the migration of these genes in the human genome.

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    • "These findings suggest that YY1 plays a crucial role in PcG complexes recruitment. Interestingly, however, a genome-wide study showed that the binding site for PcG proteins do not clearly overlap with the binding sites for YY1 [58]. More studies will be required to clarify whether transcription factors have an impact on the recruitment of PRC2 in mammals. "
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    • "The TATA and CAAT boxes are two such elements found in promoters. TATA boxes have been identified in approximately 25% of human promoters (Xi et al. 2007). They are found approximately 30 bp upstream of the TSS and recruit the transcription pre-initiation complex and RNA polymerase. "
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