Article

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.

Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Science (impact factor: 31.2). 07/2007; 316(5831):1625-8. DOI:10.1126/science.1139816 pp.1625-8
Source: PubMed

ABSTRACT Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.

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Keywords

20 million bases
 
assembly methods
 
available clone resources
 
bacterial artificial chromosome-based physical map
 
complete assembly
 
cytogenetic map
 
Drosophila heterochromatin
 
Drosophila melanogaster
 
heterochromatic regions
 
heterochromatic sequence
 
heterochromatin
 
humans
 
Mb
 
metazoans
 
nonsatellite component
 
pericentromeric heterochromatin
 
positions 11 Mb
 
sequence assembly
 
spans 13 Mb
 
specific chromosomal locations