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Development of hyaluronic acid-Fe2O3 hybrid magnetic nanoparticles for targeted delivery of peptides

Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida 33612, USA.
Nanomedicine: nanotechnology, biology, and medicine (Impact Factor: 5.98). 07/2007; 3(2):132-7. DOI: 10.1016/j.nano.2007.03.001
Source: PubMed

ABSTRACT Novel hybrid nanoparticles comprised of hyaluronic acid (HA) and iron oxide were synthesized and characterized for the first time with the average diameter of less than 160 nm. The iron oxide (Fe2O3) particles are hybridized between HA layers by electrostatic interactions between the positive surface charge of the Fe2O3 nanoparticles and the negative charge of the carboxylate groups of HA, forming a corral-like structure. The particles were also characterized by FTIR and NMR to verify the hybridization. The particles were tested for their ability to deliver peptides to the cells using HEK293 and A549 cells. Results show that these particles delivered peptides at about 100% level. These HA-iron oxide nanoparticles are expected to be useful in developing effective tissue and cell targeting systems.

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    • "c o m / l o c a t e / b b a m e m recruit HA for active targeting. Using this principle, many studies have been performed with HA–drug conjugates [8] [9] [10] or HA-targeted nanoparticles (NP) [11] [12] [13] [14] [15] [16] [17] [18]. Of the various NPs tested, liposome-based NPs are among the best studied and clinically validated [19]. "
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    • "PEG-coated MNP has the disadvantage such as limited binding sites available for further ligand binding (Gupta & Gupta, 2005), and the coating thickness can significantly affect their relaxivity (Laconte et al., 2007). In addition to PEG coating, other materials such as antibiofouling poly(TMSMA-r-PEGMA) (Lee et al., 2006), hyaluronic acid layers (Kumar et al., 2007) and carboxylfunctionalized poly(amidoamine) dendrimers of generation 3 (Shi et al., 2007) have also been used to coat the surface of IO nanoparticles for either increasing circulation time in the blood or delivering peptides at high efficiency. "
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