Therapeutic potential of human umbilical cord-derived stem cells in ischemic diseases.
ABSTRACT Recent advances suggest human umbilical cord is a new source for stem cells. Our laboratory has established a method to readily isolate and expand stem cells from human umbilical cord tissues. The aim of this study was to investigate the therapeutic potential of human umbilical cord-derived stem (UCDS) cells in ischemic diseases. The UCDS cells were characterized by flow cytometry and differentiation into osteogenic and adipogenic cells. Unilateral hind limb ischemia was surgically induced by femoral artery ligation in nude mice. The animals were intramuscularly injected with 10(6) UCDS cells or control phosphate-buffered saline. Blood perfusion of ischemic limbs was detected by laser Doppler perfusion imaging. Transplantation of UCDS cells to the ischemic limbs of nude mice significantly improved the blood flow to the affected limbs. Thus, transplantation of UCDS cells may potentially be a promising treatment for human ischemic diseases.
- SourceAvailable from: Cesar V BorlonganAdvances in Regenerative Medicine, 11/2011; , ISBN: 978-953-307-732-1
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ABSTRACT: Stem cell migration is crucial in many biological processes such as embryogenesis, histogenesis, and stem cell biology. It is also an important process in physiology, and in medicine, it can contribute to develop effective stem cell therapies. While there is a large body of experimental evidence that attempts to understand the biological and physiological mechanisms governing stem cell activity in the organism, computational modeling studies are scarce. Because stem cell migration is affected by biological diversity and the complexity of the cells’ microenvironment, experiments are hard to conduct and corresponding measurements are sophisticated. Computational modeling is a good complementary method to help us understand this process. Here, a mini-review is presented discussing the existing efforts and the unsolved key issues in stem cell migration. In addition, existing computational models studying the migration of differentiated cells are briefly discussed in the context of how they can be applied to increase our understanding of the dynamics involved in stem cell migration: Particular emphasis is placed on how biomechanical aspects of migration are explored in these models.Cellular and Molecular Bioengineering 06/2014; 7(2). · 1.23 Impact Factor
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ABSTRACT: The objective of this study was to analyze the influence of TNF-α on rat mesenchymal stem cells (MSCs) and to assess feasibility of MSC transplantation to repair ischemic injury. In this study, adhesion molecules and cell specific surface markers on MSCs were measured after exposure to different concentrations of TNF-α. MSCs stimulated with varying concentrations of TNF-α were cultured with aortic endothelial cells, and the adhesion rate was measured. MSCs were then stimulated with an optimum concentration of TNF-α as determined in vitro, and injected intravenously into rats with ischemic hind limb injury. The number of MSCs in muscle samples from the ischemic area was counted. The results showed that (1) TNF-α induced a concentration-dependent increase in VCAM-1 expression in MSCs, whereas the expression of L-selectin, ICAM-1 and VLA-4 did not change significantly. Expression of MSC-specific antigens was unchanged. (2) MSCs pretreated with 10 ng/ml TNF-α showed significantly increased adhesion to endothelial cells in vitro, and accumulated to a greater extent in the areas of ischemic damage in rat hind limbs. We were able to conclude that TNF-α has no effect on expression of MSC-specific markers, but can increase the expression of VCAM-1 on rat MSCs. Suitable concentrations of TNF-α can promote MSC adhesion to endothelial cells and migration to damaged tissue.Cell biochemistry and biophysics 11/2011; 62(3):409-14. · 3.34 Impact Factor