Therapeutic Potential of Human Umbilical Cord–Derived Stem Cells in Ischemic Diseases
Beijing Fuwai Hospital, Peping, Beijing, China Transplantation Proceedings
(Impact Factor: 0.98).
07/2007; 39(5):1620-2. DOI: 10.1016/j.transproceed.2006.12.041
Recent advances suggest human umbilical cord is a new source for stem cells. Our laboratory has established a method to readily isolate and expand stem cells from human umbilical cord tissues. The aim of this study was to investigate the therapeutic potential of human umbilical cord-derived stem (UCDS) cells in ischemic diseases. The UCDS cells were characterized by flow cytometry and differentiation into osteogenic and adipogenic cells. Unilateral hind limb ischemia was surgically induced by femoral artery ligation in nude mice. The animals were intramuscularly injected with 10(6) UCDS cells or control phosphate-buffered saline. Blood perfusion of ischemic limbs was detected by laser Doppler perfusion imaging. Transplantation of UCDS cells to the ischemic limbs of nude mice significantly improved the blood flow to the affected limbs. Thus, transplantation of UCDS cells may potentially be a promising treatment for human ischemic diseases.
Available from: Cesar V Borlongan
- "As shown in stroke investigations, the mechanisms by which HUCB stem cells protect cardiomyocytes and improve cardiac function seem to be complex and multifactorial. Transplanted HUCB cells can differentiate into cardiomyocyte-like cells (Cheng et al., 2003; Wu et al., 2006), induce neovascularization in the necrotic area (Botta et al., 2004; Ma et al., 2005; Wu et al., 2007a), modulate the inflammatory reaction induced by ischemic cascades (Henning et al., 2006), and secrete growth factors including vascular endothelial growth factor (VEGF) (Hu et al., 2006; Tang et al., 2005). However, there are some in vitro studies that suggest that HUCB stem cells in culture do not differentiate into cardiomyocytes. "
Advances in Regenerative Medicine, 11/2011; , ISBN: 978-953-307-732-1
Available from: Jonni S Moore
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ABSTRACT: Recent technological advances in flow cytometry instrumentation provide the basis for high-dimensionality and high-throughput biological experimentation in a heterogeneous cellular context. Concomitant advances in scalable computational algorithms are necessary to better utilize the information that is contained in these high-complexity experiments. The development of such tools has the potential to expand the utility of flow cytometric analysis from a predominantly hypothesis-driven mode to one of discovery, or hypothesis-generating research. A new method of analysis of flow cytometric data called Cytometric Fingerprinting (CF) has been developed. CF captures the set of multivariate probability distribution functions corresponding to list-mode data and then "flattens" them into a computationally efficient fingerprint representation that facilitates quantitative comparisons of samples. An experimental and synthetic data were generated to act as reference sets for evaluating CF. Without the introduction of prior knowledge, CF was able to "discover" the location and concentration of spiked cells in ungated analyses over a concentration range covering four orders of magnitude, to a lower limit on the order of 10 spiked events in a background of 100,000 events. We describe a new method for quantitative analysis of list-mode cytometric data. CF includes a novel algorithm for space subdivision that improves estimation of the probability density function by dividing space into nonrectangular polytopes. Additionally it renders a multidimensional distribution in the form of a one-dimensional multiresolution hierarchical fingerprint that creates a computationally efficient representation of high dimensionality distribution functions. CF supports both the generation and testing of hypotheses, eliminates sources of operator bias, and provides an increased level of automation of data analysis.
Cytometry Part A 05/2008; 73(5):430-41. DOI:10.1002/cyto.a.20545 · 2.93 Impact Factor
Available from: Wenbin Liao
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ABSTRACT: Cell-based therapy represents a promising strategy in the treatment of neurological disorders. Human umbilical cord tissue has recently been recognized as an ideal source of mesenchymal stromal cells due to accessibility, vast abundance and safety. Here, an intracerebral hemorrhage (ICH) rat model was established by injection of bacterial collagenase VII and CM-DiI labeled human umbilical cord tissue derived mesenchymal stromal cells (UC-MSC) were intracerebrally transplanted into rat brain 24 h after ICH. The results demonstrated that UC-MSC treatment significantly improved neurological function deficits and decreased injury volume of ICH rats. Leukocytes infiltration, microglial activation, ROS level and matrix metalloproteinases (MMPs) production were substantially reduced in peri-ICH area in cell-treated group as compared with PBS control at day 3 post-transplantation. In addition, UC-MSC treatment significantly increased vascular density in peri-ICH area and transplanted UC-MSC were found to be able to incorporate into cerebral vasculature in ipsilateral hemisphere at 14 days after transplantation. In summary, intracerebral administration of UC-MSC could accelerate neurological function recovery of ICH rat, the underlying mechanism may ascribe to their ability to inhibit inflammation and promote angiogenesis. Thus UC-MSC may provide a potential cell candidate for cell-based therapy in neurological disorders.
Cellular Physiology and Biochemistry 02/2009; 24(3-4):307-16. DOI:10.1159/000233255 · 2.88 Impact Factor
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