Therapeutic Potential of Human Umbilical Cord–Derived Stem Cells in Ischemic Diseases

Beijing Fuwai Hospital, Peping, Beijing, China
Transplantation Proceedings (Impact Factor: 0.98). 07/2007; 39(5):1620-2. DOI: 10.1016/j.transproceed.2006.12.041
Source: PubMed


Recent advances suggest human umbilical cord is a new source for stem cells. Our laboratory has established a method to readily isolate and expand stem cells from human umbilical cord tissues. The aim of this study was to investigate the therapeutic potential of human umbilical cord-derived stem (UCDS) cells in ischemic diseases. The UCDS cells were characterized by flow cytometry and differentiation into osteogenic and adipogenic cells. Unilateral hind limb ischemia was surgically induced by femoral artery ligation in nude mice. The animals were intramuscularly injected with 10(6) UCDS cells or control phosphate-buffered saline. Blood perfusion of ischemic limbs was detected by laser Doppler perfusion imaging. Transplantation of UCDS cells to the ischemic limbs of nude mice significantly improved the blood flow to the affected limbs. Thus, transplantation of UCDS cells may potentially be a promising treatment for human ischemic diseases.

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    • "As shown in stroke investigations, the mechanisms by which HUCB stem cells protect cardiomyocytes and improve cardiac function seem to be complex and multifactorial. Transplanted HUCB cells can differentiate into cardiomyocyte-like cells (Cheng et al., 2003; Wu et al., 2006), induce neovascularization in the necrotic area (Botta et al., 2004; Ma et al., 2005; Wu et al., 2007a), modulate the inflammatory reaction induced by ischemic cascades (Henning et al., 2006), and secrete growth factors including vascular endothelial growth factor (VEGF) (Hu et al., 2006; Tang et al., 2005). However, there are some in vitro studies that suggest that HUCB stem cells in culture do not differentiate into cardiomyocytes. "

    Advances in Regenerative Medicine, 11/2011; , ISBN: 978-953-307-732-1
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