Imaging performance of widefield solid immersion lens microscopy
ABSTRACT We investigate the performance of a widefield imaging system employing an aplanatic solid immersion lens. Off-axis imaging quality is examined theoretically at different radii and thicknesses of the aplanatic solid immersion lens. It is found that field curvature is the major aberration affecting the imaging quality. Aberrations are measured experimentally, and the results are in very good agreement with those obtained from simulations and demonstrate the situations where high quality images can be obtained with the aplanatic solid immersion lens.
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ABSTRACT: The imaging quality of an aplanatic SIL microscope is shown to be significantly degraded by aberrations, especially when the samples have thicknesses that are more than a few micrometers thicker or thinner than the design thickness. Aberration due to the sample thickness error is modeled and compared with measurements obtained in a high numerical aperture (NA ~3.5) microscope. A technique to recover near-ideal imaging quality by compensating aberrations using a MEMS deformable mirror is described and demonstrated.Optics Express 11/2013; 21(23):28189-97. DOI:10.1364/OE.21.028189 · 3.53 Impact Factor
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ABSTRACT: Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured illumination microscopy using the proximity projection grating scheme (PPGS), which has the ability to further enhance the SIM resolution without invoking any nonlinearity response from the sample. With the PPGS-based SIM, sub-100 nm resolution has been obtained experimentally, and results corresponding to 2.4 times resolution improvement are presented. Furthermore, it will be shown that an improvement of greater than 3 times can be achieved.Scientific Reports 02/2015; 5:8589. DOI:10.1038/srep08589 · 5.08 Impact Factor
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ABSTRACT: We describe a novel solid-immersion lenslet array, micro-fabricated in a chip form in the high-index (3.45) gallium phosphide. The innovatively designed lenslet features an annular aperture with appropriately patterned light absorbers and antireflection coatings. The array chip is easy to handle and enables the direct deposition of the specimen of interest onto its back-plane for tight adhesion and good optical coupling. The ensuing diffraction from the near field can yield supercritical rays inside the high-index lenslet and can, therefore, overcome the refraction and critical-angle limitations. This model showed agreement with the experimental observation of the solid-immersion fluorescence microscopy imaging, in which the refracted rays were completely blocked by the annular aperture. A large longitudinal (depth) magnification effect was also predicted and showed agreement with experiment. The annular lenslet's additional advantages of improved resolution and contrast were also discussed. Resolution of nested-L patterns with grating pitch as small as 100 nm was experimentally demonstrated. The demonstrated annular solid-immersion lenslet array concept is promising for a wider use in super-resolution optical microscopy.Journal of Applied Physics 10/2012; 112(8). DOI:10.1063/1.4761813 · 2.19 Impact Factor